The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Nm-seq maps 2′-O-methylation sites in human mRNA with base precision
Open accessAbstract
The ribose of RNA nucleotides can be 2′-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2′-O-methylated and 2′-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.
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Cites
Cutadapt removes adapter sequences from high-throughput sequencing reads
Martin, M. (2011) · EMBnet.journal · doi:10.14806/ej.17.1.200 · Cited by 33,889
BEDTools: a flexible suite of utilities for comparing genomic features
Quinlan, A. R., & Hall, I. M. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq033 · Cited by 27,437
HTSeq—a Python framework to work with high-throughput sequencing data
Anders, S., Pyl, P. T., & Huber, W. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btu638 · Cited by 21,464
Cited by
The epitranscriptome beyond m6A
Wiener, D., & Schwartz, S. (2021) · Nature Reviews Genetics · doi:10.1038/s41576-020-00295-8 · Cited by 709
METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation
Pandolfini, L., Barbieri, I., Bannister, A. J., Hendrick, A., Andrews, B., Webster, N., Murat, P., Mach, P., Brandi, R., Robson, S. C., et al. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.03.040 · Cited by 491
RMBase v2.0: deciphering the map of RNA modifications from epitranscriptome sequencing data
Xuan, J.-J., Sun, W.-J., Lin, P.-H., Zhou, K.-R., Liu, S., Zheng, L.-L., Qu, L.-H., & Yang, J.-H. (2018) · Nucleic Acids Research · doi:10.1093/nar/gkx934 · Cited by 425
The m6A epitranscriptome: transcriptome plasticity in brain development and function
Livneh, I., Moshitch-Moshkovitz, S., Amariglio, N., Rechavi, G., & Dominissini, D. (2020) · Nature Reviews Neuroscience · doi:10.1038/s41583-019-0244-z · Cited by 330
Self-citationThe proteins of mRNA modification: writers, readers, and erasers
Flamand, M. N., Tegowski, M., & Meyer, K. D. (2023) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-052521-035330 · Cited by 164
Metadata
- Authors
- Dai, Q., Moshitch-Moshkovitz, S., Han, D., Kol, N., Amariglio, N., Rechavi, G., Dominissini, D., & He, C.
- Venue
- Nature Methods
- Published
- 2017-05-15
- Volume
- 14
- Issue
- 7
- Pages
- 695-698
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2612024267
- Cited by
- 291
- References
- 3
- FWCI
- 10.00
- Type
- journal article
- Language
- en
- Open access
- green
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- Dai, Q., Moshitch-Moshkovitz, S., Han, D., Kol, N., Amariglio, N., Rechavi, G., Dominissini, D., & He, C. (2017). Nm-seq maps 2′-O-methylation sites in human mRNA with base precision. Nature Methods. https://doi.org/10.1038/nmeth.4294
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