Cited by 564Graphene and Nanomaterials ApplicationsNanoparticles: synthesis and applicationsNanotechnology research and applications

Direct imaging of single-walled carbon nanotubes in cells

Porter, Alexandra E., Gass, Mhairi, Muller, Karin, Skepper, Jeremy N., Midgley, Paul A., Welland, Mark·2007·Nature Nanotechnology

Abstract

The development of single-walled carbon nanotubes for various biomedical applications is an area of great promise. However, the contradictory data on the toxic effects of single-walled carbon nanotubes highlight the need for alternative ways to study their uptake and cytotoxic effects in cells. Single-walled carbon nanotubes have been shown to be acutely toxic in a number of types of cells, but the direct observation of cellular uptake of single-walled carbon nanotubes has not been demonstrated previously due to difficulties in discriminating carbon-based nanotubes from carbon-rich cell structures. Here we use transmission electron microscopy and confocal microscopy to image the translocation of single-walled carbon nanotubes into cells in both stained and unstained human cells. The nanotubes were seen to enter the cytoplasm and localize within the cell nucleus, causing cell mortality in a dose-dependent manner.

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Crossref · 10.1038/nnano.2007.347

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27 of 27 references verified0 citation errors · 0 not checkable

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  • MWCNTs have high toxicity and hydrophobicity; therefore CNT/SMP have to be subjected to stringent evaluation before biomedical applications.

    “For example, MWCNTs have high toxicity and hydrophobicity [562]; therefore CNT/SMP have to be subjected to stringent evaluation before biomedical applications.”
  • MWCNTs have high toxicity and hydrophobicity, so CNT/SMP have to be subjected to stringent evaluation before biomedical applications.

    “For example, MWCNTs have high toxicity and hydrophobicity [562]; therefore CNT/SMP have to be subjected to stringent evaluation before biomedical applications.”

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Authors
Porter, A. E., Gass, M., Muller, K., Skepper, J. N., Midgley, P. A., & Welland, M.
Affiliations
  • University of Cambridge (GB)
  • Daresbury Laboratory (GB)
Corresponding author
Porter, Alexandra E.
Venue
Nature Nanotechnology
Published
2007-10-28
Volume
2
Issue
11
Pages
713-717
ISSN
  • 1748-3387 (ISSN-L)
  • 1748-3395
OpenAlex
W2037122021
Cited by
564
References
28
Citations by year
2026: 1 · 2025: 5 · 2024: 14 · 2023: 10 · 2022: 22 · 2021: 8 · 2020: 14 · 2019: 19 · 2018: 65 · 2017: 28 · 2016: 34 · 2015: 31 · 2014: 44 · 2013: 47 · 2012: 54
FWCI
28.92
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Nanotechnology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Wellcome Trust
  • Leverhulme Trust
  • Engineering and Physical Sciences Research Council (EP/D040396/1)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Graphene and Nanomaterials Applications
  • Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
  • Physical Sciences › Engineering › Biomedical Engineering › Nanotechnology research and applications
Keywords
single-walled carbon nanotubes, cellular uptake, cytotoxicity, nuclear localization, dose-dependent cell death, nanotoxicology
MeSH terms
Cells, Cultured, Humans, Macrophages, Phagocytosis, Microscopy, Confocal, Nanotubes, Carbon, Microscopy, Electron, Transmission

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