The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Touchdown PCR for increased specificity and sensitivity in PCR amplification
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Citations
Cites
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Primer3 on the WWW for General Users and for Biologist Programmers
Rozen, S., & Skaletsky, H. (2003) · Bioinformatics Methods and Protocols · doi:10.1385/1-59259-192-2:365 · Cited by 15,013
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction
Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G., & Erlich, H. (1986) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.1986.051.01.032 · Cited by 3,558
A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics
SantaLucia, J. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.4.1460 · Cited by 2,793
‘Touchdown’ PCR to circumvent spurious priming during gene amplification
Don, R. H., Cox, P. T., Wainwright, B. J., Baker, K., & Mattick, J. S. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.14.4008 · Cited by 2,586
Self-citationQuantification of mRNA using real-time RT-PCR
Nolan, T., Hands, R. E., & Bustin, S. A. (2006) · Nature Protocols · doi:10.1038/nprot.2006.236 · Cited by 2,247
Chomczynski, P., & Sacchi, N. (2006) · Nature Protocols · doi:10.1038/nprot.2006.83 · Cited by 2,076
OligoCalc: an online oligonucleotide properties calculator
Kibbe, W. A. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm234 · Cited by 1,296
Cited by
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
Lorenz, T. C. (2012) · Journal of Visualized Experiments · doi:10.3791/3998 · Cited by 493
High-resolution profiling reveals coupled transcriptional and translational regulation of transgenes
Peterman, E. L., Ploessl, D. S., Love, K. S., Sanabria, V., Daniels, R. F., Johnstone, C. P., Godavarti, D. R., Kabaria, S. R., Oakes, C. G., Pai, A. A., et al. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf528 · Cited by 12
Protocol for the functional evaluation of genetic variants using saturation genome editing
Obolenski, S., Olvera-León, R., Sun, D., Adams, D. J., & Waters, A. J. (2025) · STAR Protocols · doi:10.1016/j.xpro.2025.103710 · Cited by 3
Metadata
- Authors
- Korbie, D. J., & Mattick, J. S.
- Venue
- Nature Protocols
- Published
- 2008-08-21
- Volume
- 3
- Issue
- 9
- Pages
- 1452-1456
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2159769705
- Cited by
- 624
- References
- 28
- FWCI
- 4.59
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Touchdown PCR for increased specificity and sensitivity in PCR amplification. https://app.cochat.ai/papers/10.1038/nprot.2008.133
- The paper
- Korbie, D. J., & Mattick, J. S. (2008). Touchdown PCR for increased specificity and sensitivity in PCR amplification. Nature Protocols. https://doi.org/10.1038/nprot.2008.133
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
