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A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow

Soleimani, Masoud, Nadri, Samad·2009·Nature Protocols

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Research works printed but not linked: 28

  1. Fuchs, E. & Segre, J.A. Stem cells: a new lease on life. Cell 100, 143–155 (2000). · DOI · Paper page
  2. Friedenstein, A.J., Gorskaja, J.F. & Kulagina, N.N. Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp. Hematol. 4, 267–274 (1976).
  3. Bianco, P., Riminucci, M., Gronthos, S. & Robey, P.G. Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells 19, 180–192 (2001). · DOI · Paper page
  4. Sun, S. et al. Isolation of mouse marrow mesenchymal progenitors by a novel and reliable method. Stem Cells 21, 527–535 (2003). · DOI · Paper page
  5. Prockop, D.J. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 276, 71–74 (1997). · DOI · Paper page
  6. Baksh, D., Song, L. & Tuan, R.S. Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J. Cell Mol. Med. 8, 301–316 (2004). · DOI · Paper page
  7. Horwitz, E.M. et al. Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: implications for cell therapy of bone. Proc. Natl. Acad. Sci. USA 99, 8932–8937 (2002). · DOI · Paper page
  8. Koc, O.N. et al. Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture-expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapy. J. Clin. Oncol. 18, 307–316 (2000). · DOI · Paper page
  9. Quarto, R. et al. Repair of large bone defects with the use of autologous bone marrow stromal cells. N. Engl. J. Med. 344, 385–386 (2001). · DOI · Paper page
  10. Grinnemo, K.H. et al. Xenoreactivity and engraftment of human mesenchymal stem cells transplanted into infarcted rat myocardium. J. Thorac. Cardiovasc. Surg. 127, 1293–1300 (2004). · DOI · Paper page
  11. Barry, F.P. Mesenchymal stem cell therapy in joint disease. Novartis. Found Symp. 249, 86–102, 170–4, 239–41 (2003). · DOI
  12. Peister, A. et al. Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential. Blood 103, 1662–1668 (2004). · DOI · Paper page
  13. Phinney, D.G., Kopen, G., Isaacson, R.L. & Prockop, D.J. Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation. J. Cell Biochem. 72, 570–585 (1999). · DOI · Paper page
  14. Baddoo, M. et al. Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection. J. Cell Biochem. 89, 1235–1249 (2003). · DOI · Paper page
  15. Tropel, P. et al. Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Exp. Cell Res. 295, 395–406 (2004). · DOI · Paper page
  16. Eslaminejad, M.B., Nikmahzar, A., Taghiyar, L., Nadri, S. & Massumi, M. Murine mesenchymal stem cells isolated by low density primary culture system. Dev. Growth Differ. 48, 361–370 (2006). · DOI
  17. Nadri, S. & Soleimani, M. Isolation murine mesenchymal stem cells by positive selection. In Vitro Cell Dev. Biol. Anim. 43, 276–282 (2007). · DOI
  18. Nadri, S. et al. An efficient method for isolation of murine bone marrow mesenchymal stem cells. Int. J. Dev. Biol. 51, 723–729 (2007). · DOI · Paper page
  19. Modderman, W.E., Vrijheid-Lammers, T., Lowik, C.W. & Nijweide, P.J. Removal of hematopoietic cells and macrophages from mouse bone marrow cultures: isolation of fibroblastlike stromal cells. Exp. Hematol. 22, 194–201 (1994).
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  21. Van Vlasselaer, P., Falla, N., Snoeck, H. & Mathieu, E. Characterization and purification of osteogenic cells from murine bone marrow by two-color cell sorting using anti-Sca-1 monoclonal antibody and wheat germ agglutinin. Blood 84, 753–763 (1994). · doi:10.1182/blood.v84.3.753.753
  22. Wang, Q.R. & Wolf, N.S. Dissecting the hematopoietic microenvironment. VIII. Clonal isolation and identification of cell types in murine CFU-F colonies by limiting dilution. Exp. Hematol. 18, 355–359 (1990).
  23. Kitano, Y., Radu, A., Shaaban, A. & Flake, A.W. Selection, enrichment, and culture expansion of murine mesenchymal progenitor cells by retroviral transduction of cycling adherent bone marrow cells. Exp. Hematol. 28, 1460–1469 (2000). · DOI
  24. Muraglia, A., Cancedda, R. & Quarto, R. Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model. J. Cell Sci. 113 (Part 7): 1161–1166 (2000). · DOI · Paper page
  25. Digirolamo, C.M. et al. Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate. Br. J. Haematol. 107, 275–281 (1999). · DOI · Paper page
  26. Keating, A., Horsfall, W., Hawley, R.G. & Toneguzzo, F. Effect of different promoters on expression of genes introduced into hematopoietic and marrow stromal cells by electroporation. Exp. Hematol. 18, 99–102 (1990).
  27. Deans, R.J. & Moseley, A.B. Mesenchymal stem cells: biology and potential clinical uses. Exp. Hematol. 28, 875–884 (2000). · DOI · Paper page
  28. Otsuka, T. et al. Expression of the c-kit ligand and interleukin 6 genes in mouse bone marrow stromal cell lines. Stem Cells 12, 409–415 (1994). · doi:10.1002/stem.5530120408

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Metadata

Authors
Soleimani, M., & Nadri, S.
Venue
Nature Protocols
Published
2009-01
Volume
4
Issue
1
Pages
102-106
ISSN
  • 1750-2799 (ISSN-L)
  • 1754-2189
OpenAlex
W2055171457
Cited by
909
References
2
FWCI
8.68
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)

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CoChat. (2026). Citation check: A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. https://app.cochat.ai/papers/10.1038/nprot.2008.221
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Soleimani, M., & Nadri, S. (2009). A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Nature Protocols. https://doi.org/10.1038/nprot.2008.221

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