The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Whole-mount three-dimensional imaging of internally localized immunostained cells within mouse embryos
Open accessAbstract
We describe a three-dimensional (3D) confocal imaging technique to characterize and enumerate rare, newly emerging hematopoietic cells located within the vasculature of whole-mount preparations of mouse embryos. However, the methodology is broadly applicable for examining the development and 3D architecture of other tissues. Previously, direct whole-mount imaging has been limited to external tissue layers owing to poor laser penetration of dense, opaque tissue. Our whole-embryo imaging method enables detailed quantitative and qualitative analysis of cells within the dorsal aorta of embryonic day (E) 10.5–11.5 embryos after the removal of only the head and body walls. In this protocol we describe the whole-mount fixation and multimarker staining procedure, the tissue transparency treatment, microscopy and the analysis of resulting images. A typical two-color staining experiment can be performed and analyzed in ∼6 d.
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Citations
Cites
Of lineage and legacy: the development of mammalian hematopoietic stem cells
Dzierzak, E., & Speck, N. A. (2008) · Nature Immunology · doi:10.1038/ni1560 · Cited by 707
Self-citationRequirement of Runx1/AML1/PEBP2αB for the generation of haematopoietic cells from endothelial cells
Yokomizo, T., Ogawa, M., Osato, M., Kanno, T., Yoshida, H., Fujimoto, T., Fraser, S., Nishikawa, S., Okada, H., Satake, M., et al. (2001) · Genes to Cells · doi:10.1046/j.1365-2443.2001.00393.x · Cited by 176
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Cited by
Three-dimensional imaging of solvent-cleared organs using 3DISCO
Ertürk, A., Becker, K., Jährling, N., Mauch, C. P., Hojer, C. D., Egen, J. G., Hellal, F., Bradke, F., Sheng, M., & Dodt, H.-U. (2012) · Nature Protocols · doi:10.1038/nprot.2012.119 · Cited by 992
Deep imaging of bone marrow shows non-dividing stem cells are mainly perisinusoidal
Acar, M., Kocherlakota, K. S., Murphy, M. M., Peyer, J. G., Oguro, H., Inra, C. N., Jaiyeola, C., Zhao, Z., Luby-Phelps, K., & Morrison, S. J. (2015) · Nature · doi:10.1038/nature15250 · Cited by 696
Inflammatory signaling regulates embryonic hematopoietic stem and progenitor cell production
Li, Y., Esain, V., Teng, L., Xu, J., Kwan, W., Frost, I. M., Yzaguirre, A. D., Cai, X., Cortes, M., Maijenburg, M. W., et al. (2014) · Genes & Development · doi:10.1101/gad.253302.114 · Cited by 261
Self-citationGata2 is required for HSC generation and survival
de Pater, E., Kaimakis, P., Vink, C. S., Yokomizo, T., Yamada-Inagawa, T., van der Linden, R., Kartalaei, P. S., Camper, S. A., Speck, N., & Dzierzak, E. (2013) · Journal of Experimental Medicine · doi:10.1084/jem.20130751 · Cited by 257
Self-citationACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging
Lee, E., Choi, J., Jo, Y., Kim, J. Y., Jang, Y. J., Lee, H. M., Kim, S. Y., Lee, H.-J., Cho, K., Jung, N., et al. (2016) · Scientific Reports · doi:10.1038/srep18631 · Cited by 215
Metadata
- Authors
- Yokomizo, T., Yamada-Inagawa, T., Yzaguirre, A. D., Chen, M. J., Speck, N. A., & Dzierzak, E.
- Venue
- Nature Protocols
- Published
- 2012-02-09
- Volume
- 7
- Issue
- 3
- Pages
- 421-431
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2069314780
- Cited by
- 164
- References
- 2
- FWCI
- 5.90
- Type
- journal article
- Language
- en
- Open access
- green
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