The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Cell cycle staging of individual cells by fluorescence microscopy
Open accessAbstract
Progression through the cell cycle is one of the most fundamental features of cells. Studies of the cell cycle have traditionally relied on the analysis of populations, and they often require specific markers or the use of genetically modified systems, making it difficult to determine the cell cycle stage of individual, unperturbed cells. We describe a protocol, suitable for use in high-resolution imaging approaches, for determining cell cycle staging of individual cells by measuring their DNA content by fluorescence microscopy. The approach is based on the accurate quantification by image analysis of the integrated nuclear intensity of cells stained with a DNA dye, and it can be used in combination with several histochemical methods. We describe and provide the algorithms for two automated image analysis pipelines and the derivation of cell cycle profiles with both commercial and open-source software. This 1–2-d protocol is applicable to adherent cells, and it is adaptable for use with several DNA dyes.
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ggplot2: Elegant Graphics for Data Analysis
Wickham, H. (2009) · doi:10.1007/978-0-387-98141-3 · Cited by 14,447
Cellular senescence: when bad things happen to good cells
Campisi, J., & d'Adda di Fagagna, F. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2233 · Cited by 4,441
Cell cycle, CDKs and cancer: a changing paradigm
Malumbres, M., & Barbacid, M. (2009) · Nature Reviews Cancer · doi:10.1038/nrc2602 · Cited by 3,957
Regulation of DNA repair throughout the cell cycle
Branzei, D., & Foiani, M. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2351 · Cited by 1,228
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Zirkel, A., Nikolic, M., Sofiadis, K., Mallm, J.-P., Brackley, C. A., Gothe, H., Drechsel, O., Becker, C., Altmüller, J., Josipovic, N., et al. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.03.030 · Cited by 226
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Little, A. C., Kovalenko, I., Goo, L. E., Hong, H. S., Kerk, S. A., Yates, J. A., Purohit, V., Lombard, D. B., Merajver, S. D., & Lyssiotis, C. A. (2020) · Communications Biology · doi:10.1038/s42003-020-0988-z · Cited by 88
Metadata
- Authors
- Roukos, V., Pegoraro, G., Voss, T. C., & Misteli, T.
- Venue
- Nature Protocols
- Published
- 2015-01-29
- Volume
- 10
- Issue
- 2
- Pages
- 334-348
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W1994363917
- Cited by
- 194
- References
- 4
- FWCI
- 8.10
- Type
- journal article
- Language
- en
- Open access
- green
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