The DOI matches its Crossref record; 179 of the 181 references it cites were found in a registry or an index.
Design and development of polymers for gene delivery
Abstract
The lack of safe and efficient gene-delivery methods is a limiting obstacle to human gene therapy. Synthetic gene-delivery agents, although safer than recombinant viruses, generally do not possess the required efficacy. In recent years, a variety of effective polymers have been designed specifically for gene delivery, and much has been learned about their structure-function relationships. With the growing understanding of polymer gene-delivery mechanisms and continued efforts of creative polymer chemists, it is likely that polymer-based gene-delivery systems will become an important tool for human gene therapy.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“As an alternative, non-viral vectors may offer tantalizing possibility in CRISPR/Cas9 delivery with respect to their low immunogenicity, absence of endogenous virus recombination, less limitation in delivering larger genetic payloads and ease of large-scale production [73–75].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [73]
In the citing paper
“There are two main limitations of non-viral vectors-mediated CRISPR/Cas9 delivery in vivo. The first one is the serum stability of polyplexes after systemic administration [73]. The second one is the relatively low transfection efficiency in the targeted tissues after administration in vivo [76, 77].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [73]
In the citing paper
“Besides, in contrast with viral vectors, cumulative editing efficacy can be obtained after multiple administrations of non-viral vectors-mediated CRISPR/Cas9 delivery owing to limited immune responses [73, 74, 116, 138].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [73]
In the citing paper
“Nanoparticles smaller than 9 nm can passively diffuse through nuclear pore complexes (NPCs), while larger ones requiring the assistance of import proteins (e.g., importins) to actively shuttle them into the nucleus [73].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [73]
Citations
Cites
Boussif, O., Lezoualc’h, F., Zanta, M. A., Djavaheri‐Mergny, M., Scherman, D., DEMENEIX, B. A., & Behr, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7297 · Cited by 6,256
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Felgner, P. L., Gadek, T. R., Holm, M., Roman, R., Chan, H. W., Wenz, M., Northrop, J. P., RINGOLD, G. M., & Danielsen, M. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7413 · Cited by 4,954
Tomalia, D. A., Naylor, A. M., & Goddard, W. A. (1990) · Angewandte Chemie International Edition in English · doi:10.1002/anie.199001381 · Cited by 3,222
Receptor Binding and Membrane Fusion in Virus Entry: The Influenza Hemagglutinin
Skehel, J. J., & Wiley, D. C. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.531 · Cited by 2,706
Gene Therapy of Human Severe Combined Immunodeficiency (SCID)-X1 Disease
Cavazzana, M., Hacein‐Bey‐Abina, S., Basile, G. D. S., Gross, F., Yvon, E. S., Nusbaum, P., Selz, F., Hue, C., Dogniaux, S., Casanova, J., et al. (2000) · Science · doi:10.1126/science.288.5466.669 · Cited by 2,685
Cyclodextrin-based pharmaceutics: past, present and future
Davis, M. E., & Brewster, M. E. (2004) · Nature Reviews Drug Discovery · doi:10.1038/nrd1576 · Cited by 2,057
The Basic Science of Gene Therapy
Mulligan, R. C. (1993) · Science · doi:10.1126/science.8493530 · Cited by 1,741
Gene therapy - promises, problems and prospects
Verma, I. M., & Somia, N. (1997) · Nature · doi:10.1038/38410 · Cited by 1,681
T Lymphocyte-Directed Gene Therapy for ADA − SCID: Initial Trial Results After 4 Years
Blaese, R. M., Culver, K. W., Miller, A. D., Carter, C. S., Fleisher, T. A., Clerici, M., Shearer, G. M., Chang, L., Chiang, Y. L., Tolstoshev, P., et al. (1995) · Science · doi:10.1126/science.270.5235.475 · Cited by 1,546
Mukherjee, S., Ghosh, R. K., & Maxfield, F. R. (1997) · Physiological Reviews · doi:10.1152/physrev.1997.77.3.759 · Cited by 1,338
Research works printed but not linked: 4
- Schaffer, D. V. in Chemical Engineering 125 (Massachusetts Institute of Technology, Cambridge, Massachusetts, 1998).
- Wilson, G., Dean, B. S., Wang, G. & Dean, D. A. DNA vector chemistry: the covalent attachment of signal peptides to plasmid DNA. J. Biol. Chem. 16, 80–85 (1998).
- Henry, S., Pirie, C., Stayton, P. S. & Hoffman, A. S. Membrane–disruption ability of maleic anhydride copolymers at endosomal pHs. Biomacromolecules (in the press).
- El-Sayed, M., Hoffman, A. S. & Stayton, P. S. Influence of composition of novel pH-sensitive and glutathione-reactive polymeric carriers on their membrane-destabilizing activity. J. Control. Release (in the press).
Cited by
Sorted by citations
Non-viral vectors for gene-based therapy
Yin, H., Kanasty, R. L., Eltoukhy, A. A., Vegas, A. J., Dorkin, J. R., & Anderson, D. G. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3763 · Cited by 3,123
Polymer conjugates as anticancer nanomedicines
Duncan, R. (2006) · Nature reviews. Cancer · doi:10.1038/nrc1958 · Cited by 1,956
Mucus-penetrating nanoparticles for drug and gene delivery to mucosal tissues
Lai, S. K., Wang, Y.-Y., & Hanes, J. S. (2008) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2008.11.002 · Cited by 1,863
Polymers for Drug Delivery Systems
Liechty, W. B., Kryscio, D. R., Slaughter, B. V., & Peppas, N. A. (2010) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-073009-100847 · Cited by 1,608
Block copolymer micelles in nanomedicine applications
Cabral, H., Miyata, K., Osada, K., & Kataoka, K. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00199 · Cited by 1,309
Metadata
- Authors
- Pack, D. W., Hoffman, A. S., Pun, S., & Stayton, P. S.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- University of Washington (US)
- Corresponding author
- Pack, Daniel W.
- Venue
- Nature Reviews Drug Discovery
- Published
- 2005-07-01
- Volume
- 4
- Issue
- 7
- Pages
- 581-593
- ISSN
- 1474-1776 (ISSN-L)
- 1474-1784
- OpenAlex
- W2003414091
- Cited by
- 2,568
- References
- 181
- Citations by year
- 2026: 55 · 2025: 67 · 2024: 94 · 2023: 76 · 2022: 109 · 2021: 122 · 2020: 125 · 2019: 144 · 2018: 159 · 2017: 157 · 2016: 173 · 2015: 205 · 2014: 180 · 2013: 180 · 2012: 188
- FWCI
- 4.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Reviews Drug Discovery (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- gene therapy, structure-function relationship, polymer chemistry, non-viral vectors
- MeSH terms
- Animals, Cyclodextrins, Genetic Vectors, Humans, Imidazoles, Polymers, Drug Design, Genetic Therapy, Gene Transfer Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
