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Cited by 76Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchLiver physiology and pathology

Application of whole-organ tissue engineering in hepatology

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Uygun, Basak E., Yarmush, Martin L., Uygun, Korkut·2012·Nature Reviews Gastroenterology & Hepatology
DOI 10.1038/nrgastro.2012.140PMC PMC3732057
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Abstract

Initially hailed as the ultimate solution to organ failure, engineering of vascularized tissues such as the liver has stalled because of the need for a well-structured circulatory system that can maintain the cells seeded inside the construct. A new approach has evolved to overcome this obstacle. Whole-organ decellularization is a method that retains most of the native vascular structures of the organ, providing microcirculatory support and structure, which can be anastomosed with the recipient circulation. The technique was first applied to the heart and then adapted for the liver. Several studies have shown that cells can be eliminated, the extracellular matrix and vasculature are reasonably preserved and, after repopulation with hepatocytes, these grafts can perform hepatic functions in vitro and in vivo. Progress is rapidly being made as researchers are addressing several key challenges to whole-organ tissue engineering, such as ensuring correct cell distribution, nonparenchymal cell seeding, blood compatibility, immunological concerns, and the source of cells and matrices.

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Authors
Uygun, B. E., Yarmush, M. L., & Uygun, K.
Affiliations
Harvard University (US)
Corresponding author
Uygun, Korkut
ORCID
Venue
Nature Reviews Gastroenterology & Hepatology
Publisher
Springer Science and Business Media LLC
Published
2012-08-14
Volume
9
Issue
12
Pages
738-744
ISSN
  • 1759-5045 (ISSN-L)
  • 1759-5053
DOI
10.1038/nrgastro.2012.140
PMC
PMC3732057
OpenAlex
W2044046384
Cited by
76
References
5
Citations by year
2025: 6 · 2024: 3 · 2023: 6 · 2022: 2 · 2021: 6 · 2020: 3 · 2019: 8 · 2018: 4 · 2017: 8 · 2016: 8 · 2015: 11 · 2014: 8 · 2013: 3
FWCI
1.36
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature Reviews Gastroenterology & Hepatology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Science Foundation
  • Shriners Hospitals for Children
  • National Institute of Diabetes and Digestive and Kidney Diseases (K99DK088962, R01DK084053, R01DK096075R01, R01DK096075, R00DK088962)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords
liver regeneration, decellularization, recellularization, donor liver shortage, hepatocyte repopulation, extracellular matrix, vascularization, organ transplantation, hemocompatibility, cell seeding
MeSH terms
Animals, Disease Models, Animal, Extracellular Matrix, Gastroenterology, Liver, Microcirculation, Liver Failure, Hepatocytes, Tissue Engineering, Mice

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