Hemocompatibility improvement of perfusion-decellularized clinical-scale liver scaffold through heparin immobilization
Open accessAbstract
Whole-liver perfusion-decellularization is an attractive scaffold-preparation technique for producing clinical transplantable liver tissue. However, the scaffold's poor hemocompatibility poses a major obstacle. This study was intended to improve the hemocompatibility of perfusion-decellularized porcine liver scaffold via immobilization of heparin. Heparin was immobilized on decellularized liver scaffolds (DLSs) by electrostatic binding using a layer-by-layer self-assembly technique (/h-LBL scaffold), covalent binding via multi-point attachment (/h-MPA scaffold), or end-point attachment (/h-EPA scaffold). The effect of heparinization on anticoagulant ability and cytocompatibility were investigated. The result of heparin content and release tests revealed EPA technique performed higher efficiency of heparin immobilization than other two methods. Then, systematic in vitro investigation of prothrombin time (PT), thrombin time (TT), activated partial thromboplastin time (APTT), platelet adhesion and human platelet factor 4 (PF4, indicates platelet activation) confirmed the heparinized scaffolds, especially the /h-EPA counterparts, exhibited ultralow blood component activations and excellent hemocompatibility. Furthermore, heparin treatments prevented thrombosis successfully in DLSs with blood perfusion after implanted in vivo. Meanwhile, after heparin processes, both primary hepatocyte and endothelial cell viability were also well-maintained, which indicated that heparin treatments with improved biocompatibility might extend to various hemoperfusable whole-organ scaffolds' preparation.
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In a clinical-scale porcine liver, heparin treatments effectively prevented thrombosis in the bioengineered liver during blood perfusion after in vivo implantation.
“They observed that heparin treatments effectively prevented thrombosis in the bioengineered liver during blood perfusion after in vivo implantation.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [219]
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Self-citation
- Authors
- Bao, J., Wu, Q., Sun, J., Zhou, Y., Wang, Y., Jiang, X., Li, L., Shi, Y., & Bu, H.
- Affiliations
- Sichuan University (CN)
- Corresponding author
- Bu, Hong
- Venue
- Scientific Reports
- Published
- 2015-06-01
- Volume
- 5
- Issue
- 1
- Pages
- 10756-10756
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5377232
- OpenAlex
- W2261148770
- Cited by
- 82
- References
- 32
- Citations by year
- 2026: 7 · 2025: 1 · 2024: 5 · 2023: 9 · 2022: 5 · 2021: 11 · 2020: 7 · 2019: 8 · 2018: 12 · 2017: 7 · 2016: 9 · 2015: 1
- FWCI
- 9.74
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- China Postdoctoral Science Foundation (2011M501413, 2013T60855, 81200315, 2011SCU11044)
- Sichuan University (2011SCU11044, 2013T60855, 2011M501413, 81200315)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- hemocompatibility, decellularized liver scaffold, heparin immobilization, perfusion decellularization, porcine liver, anticoagulant activity, platelet activation, thromboprophylaxis, layer-by-layer assembly, covalent binding, electrostatic binding, hepatocyte viability, endothelial cell viability, liver transplantation, blood perfusion
- MeSH terms
- Animals, Anticoagulants, Antigens, Blood Platelets, Heparin, Liver, Perfusion, Platelet Aggregation, Swine, Platelet Activation, Cell Transplantation, Tissue Engineering, Tissue Scaffolds
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