Mesenchymal stem cells support hepatocyte function in engineered liver grafts
Open accessAbstract
Recent studies suggest that organ decellularization is a promising approach to facilitate the clinical application of regenerative therapy by providing a platform for organ engineering. This unique strategy uses native matrices to act as a reservoir for the functional cells which may show therapeutic potential when implanted into the body. Appropriate cell sources for artificial livers have been debated for some time. The desired cell type in artificial livers is primary hepatocytes, but in addition, other supportive cells may facilitate this stem cell technology. In this context, the use of mesenchymal stem cells (MSC) is an option meeting the criteria for therapeutic organ engineering. Ideally, supportive cells are required to (1) reduce the hepatic cell mass needed in an engineered liver by enhancing hepatocyte function, (2) modulate hepatic regeneration in a paracrine fashion or by direct contact, and (3) enhance the preservability of parenchymal cells during storage. Here, we describe enhanced hepatic function achieved using a strategy of sequential infusion of cells and illustrate the advantages of co-cultivating bone marrow-derived MSCs with primary hepatocytes in the engineered whole-liver scaffold. These co-recellularized liver scaffolds colonized by MSCs and hepatocytes were transplanted into live animals. After blood flow was established, we show that expression of adhesion molecules and proangiogenic factors was upregulated in the graft.
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Enzymatic treatment, particularly involving nucleases and trypsin, is often combined with chemical detergents followed by various washing cycles for liver decellularization.
“Hence, enzymatic treatment, particularly involving nucleases and trypsin, is often combined with chemical detergents followed by various washing cycles for liver decellularization [110,123,149,150].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [123]
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- Authors
- Kadota, Y., Yagi, H., Inomata, K., Matsubara, K., Hibi, T., Abe, Y., Kitago, M., Shinoda, M., Obara, H., Itano, O., & Kitagawa, Y.
- Affiliations
- Keio University (JP)
- Corresponding author
- Yagi, Hiroshi
- ORCID
- Venue
- Organogenesis
- Publisher
- Informa UK Limited
- Published
- 2014-01-31
- Volume
- 10
- Issue
- 2
- Pages
- 268-277
- ISSN
- 1547-6278 (ISSN-L)
- 1555-8592
- PMC
- PMC4154962
- OpenAlex
- W2142076588
- Cited by
- 58
- References
- 50
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 3 · 2019: 12 · 2018: 9 · 2017: 4 · 2016: 10 · 2015: 5 · 2014: 2
- FWCI
- 6.95
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Organogenesis (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Takeda Science Foundation
- Research Center Network for Realization of Regenerative Medicine
- Keio University
- Japan Society for the Promotion of Science (23689059)
- Japan Science and Technology Agency
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- mesenchymal stem cells, hepatocyte function, bone marrow-derived stem cells, artificial liver, liver regeneration, paracrine signaling, co-culture, adhesion molecules, angiogenic factors, cell storage
- MeSH terms
- Animals, Cell Count, Female, Liver, Rats, Inbred Lew, Cell Adhesion Molecules, Liver Transplantation, Hepatocytes, Tissue Engineering, Angiogenesis Inducing Agents, Tissue Scaffolds, Mesenchymal Stem Cells
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