The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
The splicing factor SC35 has an active role in transcriptional elongation
Open accessAbstract
Mounting evidence suggests that transcription and RNA processing are intimately coupled in vivo, although each process can occur independently in vitro. It is generally thought that polymerase II (Pol II) C-terminal domain (CTD) kinases are recruited near the transcription start site to overcome initial Pol II pausing events, and that stably bound kinases facilitate productive elongation and co-transcriptional RNA processing. Whereas most studies have focused on how RNA processing machineries take advantage of the transcriptional apparatus to efficiently modify nascent RNA, here we report that a well-studied splicing factor, SC35, affects transcriptional elongation in a gene-specific manner. SC35 depletion induces Pol II accumulation within the gene body and attenuated elongation, which are correlated with defective P-TEFb (a complex composed of CycT1–CDK9) recruitment and dramatically reduced CTD Ser2 phosphorylation. Recombinant SC35 is sufficient to rescue this defect in nuclear run-on experiments. These findings suggest a reciprocal functional relationship between the transcription and splicing machineries during gene expression.
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Cites
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Self-citationElongation by RNA polymerase II: the short and long of it
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Self-citationAlternative splicing: a pivotal step between eukaryotic transcription and translation
Kornblihtt, A. R., Schor, I. E., Alló, M., Dujardin, G., Petrillo, E., & Muñoz, M. J. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3525 · Cited by 853
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Metadata
- Authors
- Lin, S., Coutinho-Mansfield, G., Wang, D., Pandit, S., & Fu, X.-D.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2008-07-20
- Volume
- 15
- Issue
- 8
- Pages
- 819-826
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W1996505914
- Cited by
- 362
- References
- 52
- FWCI
- 11.29
- Type
- journal article
- Language
- en
- Open access
- green
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- Lin, S., Coutinho-Mansfield, G., Wang, D., Pandit, S., & Fu, X.-D. (2008). The splicing factor SC35 has an active role in transcriptional elongation. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.1461
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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