Cited by 192Genomics and Chromatin DynamicsEpigenetics and DNA MethylationChromatin Remodeling and Cancer

The histone variant macroH2A is an epigenetic regulator of key developmental genes

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Buschbeck, Marcus, Uribesalgo, Iris, Wibowo, Indra, Rué, Pau, Martin, David, Gutierrez, Arantxa, Morey, Lluís, Guigó, Roderic +2 more·2009·Nature Structural & Molecular Biology

Abstract

The histone variants macroH2A1 and macroH2A2 are associated with X chromosome inactivation in female mammals. However, the physiological function of macroH2A proteins on autosomes is poorly understood. Microarray-based analysis in human male pluripotent cells uncovered occupancy of both macroH2A variants at many genes encoding key regulators of development and cell fate decisions. On these genes, the presence of macroH2A1+2 is a repressive mark that overlaps locally and functionally with Polycomb repressive complex 2. We demonstrate that macroH2A1+2 contribute to the fine-tuning of temporal activation of HOXA cluster genes during neuronal differentiation. Furthermore, elimination of macroH2A2 function in zebrafish embryos produced severe but specific phenotypes. Taken together, our data demonstrate that macroH2A variants constitute an important epigenetic mark involved in the concerted regulation of gene expression programs during cellular differentiation and vertebrate development.

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Crossref · 10.1038/nsmb.1665

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  • Certain mRNAs are less m6A methylated in old versus young human blood cells.

    “In addition to changes in DNA and histone methylation, certain mRNAs are less m6A methylated in old versus young human blood cells 72 .”

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Authors
Buschbeck, M., Uribesalgo, I., Wibowo, I., Rué, P., Martin, D., Gutierrez, A., Morey, L., Guigó, R., López-Schier, H., & Di Croce, L.
Affiliations
Barcelona Biomedical Research Park (ES)
Venue
Nature Structural & Molecular Biology
Published
2009-09-06
Volume
16
Issue
10
Pages
1074-1079
ISSN
  • 1545-9985 (ISSN-L)
  • 1545-9993
OpenAlex
W2038356000
Cited by
192
References
41
Citations by year
2026: 2 · 2025: 7 · 2024: 10 · 2023: 5 · 2022: 7 · 2021: 11 · 2020: 8 · 2019: 10 · 2018: 10 · 2017: 13 · 2016: 15 · 2015: 11 · 2014: 19 · 2013: 20 · 2012: 17
FWCI
5.90
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Structural & Molecular Biology (publishedVersion)
  • PubMed (publishedVersion)
  • UPCommons institutional repository (Universitat Politècnica de Catalunya) (acceptedVersion)
  • Site cant be reached (submittedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
  • RECERCAT (Consorci de Serveis Universitaris de Catalunya) (submittedVersion, public-domain)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
Keywords
macroH2A, histone variants, epigenetic regulation, gene repression, polycomb repressive complex 2, HOXA cluster, neuronal differentiation, zebrafish embryonic development, X chromosome inactivation, pluripotent cells, developmental gene regulation, chromatin modification
MeSH terms
Animals, Gene Expression Regulation, Multigene Family, Histones, Humans, Male, Phenotype, Repressor Proteins, Stem Cells, Genetic Variation, Zebrafish, Homeodomain Proteins, Cell Lineage, Oligonucleotide Array Sequence Analysis, Epigenesis, Genetic, Polycomb-Group Proteins

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