The histone variant macroH2A is an epigenetic regulator of key developmental genes
Open accessAbstract
The histone variants macroH2A1 and macroH2A2 are associated with X chromosome inactivation in female mammals. However, the physiological function of macroH2A proteins on autosomes is poorly understood. Microarray-based analysis in human male pluripotent cells uncovered occupancy of both macroH2A variants at many genes encoding key regulators of development and cell fate decisions. On these genes, the presence of macroH2A1+2 is a repressive mark that overlaps locally and functionally with Polycomb repressive complex 2. We demonstrate that macroH2A1+2 contribute to the fine-tuning of temporal activation of HOXA cluster genes during neuronal differentiation. Furthermore, elimination of macroH2A2 function in zebrafish embryos produced severe but specific phenotypes. Taken together, our data demonstrate that macroH2A variants constitute an important epigenetic mark involved in the concerted regulation of gene expression programs during cellular differentiation and vertebrate development.
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Certain mRNAs are less m6A methylated in old versus young human blood cells.
“In addition to changes in DNA and histone methylation, certain mRNAs are less m6A methylated in old versus young human blood cells 72 .”
From The roles of DNA, RNA and histone methylation in ageing and cancer (2019) · cites this paper as [72]
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- Authors
- Buschbeck, M., Uribesalgo, I., Wibowo, I., Rué, P., Martin, D., Gutierrez, A., Morey, L., Guigó, R., López-Schier, H., & Di Croce, L.
- Affiliations
- Barcelona Biomedical Research Park (ES)
- Venue
- Nature Structural & Molecular Biology
- Published
- 2009-09-06
- Volume
- 16
- Issue
- 10
- Pages
- 1074-1079
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W2038356000
- Cited by
- 192
- References
- 41
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 10 · 2023: 5 · 2022: 7 · 2021: 11 · 2020: 8 · 2019: 10 · 2018: 10 · 2017: 13 · 2016: 15 · 2015: 11 · 2014: 19 · 2013: 20 · 2012: 17
- FWCI
- 5.90
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- public-domain
- www.springer.com/tdm (tdm)
- Locations
- Nature Structural & Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- UPCommons institutional repository (Universitat Politècnica de Catalunya) (acceptedVersion)
- Site cant be reached (submittedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- RECERCAT (Consorci de Serveis Universitaris de Catalunya) (submittedVersion, public-domain)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Keywords
- macroH2A, histone variants, epigenetic regulation, gene repression, polycomb repressive complex 2, HOXA cluster, neuronal differentiation, zebrafish embryonic development, X chromosome inactivation, pluripotent cells, developmental gene regulation, chromatin modification
- MeSH terms
- Animals, Gene Expression Regulation, Multigene Family, Histones, Humans, Male, Phenotype, Repressor Proteins, Stem Cells, Genetic Variation, Zebrafish, Homeodomain Proteins, Cell Lineage, Oligonucleotide Array Sequence Analysis, Epigenesis, Genetic, Polycomb-Group Proteins
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