The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis
Open accessAbstract
RNA interference pathways can involve amplification of secondary siRNAs by RNA-dependent RNA polymerases. In plants, RDR6-dependent secondary siRNAs arise from transcripts targeted by some microRNAs (miRNAs). Here, Arabidopsis thaliana secondary siRNAs from mRNA as well as trans-acting siRNAs are shown to be triggered through initial targeting by a 22-nucleotide (nt) miRNA that associates with AGO1. In contrast to canonical 21-nt miRNAs, 22-nt miRNAs primarily arise from foldback precursors containing asymmetric bulges. Using artificial miRNA constructs, conversion of asymmetric foldbacks to symmetric foldbacks resulted in the production of 21-nt forms of miR173, miR472 and miR828. Both 21- and 22-nt forms associated with AGO1 and guided accurate slicer activity, but only 22-nt forms were competent to trigger RDR6-dependent siRNA production from target RNA. These data suggest that AGO1 functions differentially with 21- and 22-nt miRNAs to engage the RDR6-associated amplification apparatus.
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Cites
Cleavage of Scarecrow-like mRNA Targets Directed by a Class of Arabidopsis miRNA
Llave, C., Xie, Z., Kasschau, K. D., & Carrington, J. C. (2002) · Science · doi:10.1126/science.1076311 · Cited by 1,627
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Cited by
psRNATarget: a plant small RNA target analysis server (2017 release)
Dai, X., Zhuang, Z., & Zhao, P. X. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky316 · Cited by 1,449
Araport11: a complete reannotation of the Arabidopsis thaliana reference genome
Cheng, C., Krishnakumar, V., Chan, A. P., Thibaud‐Nissen, F., Schobel, S., & Town, C. D. (2017) · The Plant Journal · doi:10.1111/tpj.13415 · Cited by 1,317
Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs
Rogers, K., & Chen, X. (2013) · The Plant Cell · doi:10.1105/tpc.113.113159 · Cited by 935
MicroRNA regulation of plant innate immune receptors
Li, F., Pignatta, D., Bendix, C., Brunkard, J. O., Cohn, M. M., Tung, J., Sun, H., Kumar, P., & Baker, B. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1118282109 · Cited by 616
The ‘how’ and ‘where’ of plant micro RNA s
Yu, Y., Jia, T., & Chen, X. (2017) · New Phytologist · doi:10.1111/nph.14834 · Cited by 459
Metadata
- Authors
- Cuperus, J. T., Carbonell, A., Fahlgren, N., Garcia-Ruiz, H., Burke, R. T., Takeda, A., Sullivan, C. M., Gilbert, S. D., Montgomery, T. A., & Carrington, J. C.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2010-06-18
- Volume
- 17
- Issue
- 8
- Pages
- 997-1003
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W2141548533
- Cited by
- 447
- References
- 1
- FWCI
- 45.11
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Cuperus, J. T., Carbonell, A., Fahlgren, N., Garcia-Ruiz, H., Burke, R. T., Takeda, A., Sullivan, C. M., Gilbert, S. D., Montgomery, T. A., & Carrington, J. C. (2010). Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.1866
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