The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
CYP6AE gene cluster knockout in Helicoverpa armigera reveals role in detoxification of phytochemicals and insecticides
Open accessAbstract
The cotton bollworm Helicoverpa armigera, is one of the world’s major pest of agriculture, feeding on over 300 hosts in 68 plant families. Resistance cases to most insecticide classes have been reported for this insect. Management of this pest in agroecosystems relies on a better understanding of how it copes with phytochemical or synthetic toxins. We have used genome editing to knock out a cluster of nine P450 genes and show that this significantly reduces the survival rate of the insect when exposed to two classes of host plant chemicals and two classes of insecticides. Functional expression of all members of this gene cluster identified the P450 enzymes capable of metabolism of these xenobiotics. The CRISPR-Cas9-based reverse genetics approach in conjunction with in vitro metabolism can rapidly identify the contributions of insect P450s in xenobiotic detoxification and serve to identify candidate genes for insecticide resistance.
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Cites
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Self-citationInsect CYP Genes and P450 Enzymes
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Cited by
The Role of Cytochrome P450s in Insect Toxicology and Resistance
Nauen, R., Bass, C., Feyereisen, R., & Vontas, J. (2022) · Annual Review of Entomology · doi:10.1146/annurev-ento-070621-061328 · Cited by 508
Self-citationSynergistic insights into pesticide persistence and microbial dynamics for bioremediation
Ray, S. S., Parihar, K., Goyal, N., & Mahapatra, D. M. (2024) · Environmental Research · doi:10.1016/j.envres.2024.119290 · Cited by 37
Metadata
- Authors
- Wang, H., Shi, Y., Wang, L., Liu, S., Wu, S., Yang, Y., Feyereisen, R., & Wu, Y.
- Venue
- Nature Communications
- Published
- 2018-11-16
- Volume
- 9
- Issue
- 1
- Pages
- 4820-4820
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2901126365
- Cited by
- 299
- References
- 38
- FWCI
- 6.59
- Type
- journal article
- Language
- en
- Open access
- gold
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- Wang, H., Shi, Y., Wang, L., Liu, S., Wu, S., Yang, Y., Feyereisen, R., & Wu, Y. (2018). CYP6AE gene cluster knockout in Helicoverpa armigera reveals role in detoxification of phytochemicals and insecticides. Nature Communications. https://doi.org/10.1038/s41467-018-07226-6
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