Subcellular connectomic analyses of energy networks in striated muscle
Open accessAbstract
Mapping biological circuit connectivity has revolutionized our understanding of structure-function relationships. Although connectomic analyses have primarily focused on neural systems, electrical connectivity within muscle mitochondrial networks was recently demonstrated to provide a rapid mechanism for cellular energy distribution. However, tools to evaluate organelle connectivity with high spatial fidelity within single cells are currently lacking. Here, we developed a framework to quantitatively assess mitochondrial network connectivity and interactions with cellular sites of energy storage, utilization, and calcium cycling in cardiac, oxidative, and glycolytic muscle. We demonstrate that mitochondrial network configuration, individual mitochondrial size and shape, and the junctions connecting mitochondria within each network are consistent with the differing contraction demands of each muscle type. Moreover, mitochondria-lipid droplet interaction analyses suggest that individual mitochondria within networks may play specialized roles regarding energy distribution and calcium cycling within the cell and reveal the power of connectomic analyses of organelle interactions within single cells.
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The mitochondrial network morphology, arrangement, and connectivity adapt to each fiber type's specific functional needs (e.g., OXPHOS capacity and contractility).
“Therefore, the mitochondrial network morphology, arrangement, and connectivity adapt to each fiber type's specific functional needs (e.g., OXPHOS capacity and contractility) [31, 32].”
From The connection between the dynamic remodeling of the mitochondrial network and the regulation of muscle mass (2021) · cites this paper as [32]
Mitochondrial fusion leads to elongated organelles, which expand the mitochondrial network and its interconnectivity.
“Mitochondrial fusion leads to elongated organelles, which expand the mitochondrial network and its interconnectivity [32].”
From The connection between the dynamic remodeling of the mitochondrial network and the regulation of muscle mass (2021) · cites this paper as [32]
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- Authors
- Bleck, C. K. E., Kim, Y., Willingham, T. B., & Glancy, B.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Glancy, Brian
- Venue
- Nature Communications
- Published
- 2018-11-30
- Volume
- 9
- Issue
- 1
- Pages
- 5111-5111
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC6269443
- OpenAlex
- W2903334028
- Cited by
- 150
- References
- 52
- Citations by year
- 2026: 14 · 2025: 19 · 2024: 19 · 2023: 22 · 2022: 20 · 2021: 21 · 2020: 23 · 2019: 12
- FWCI
- 4.98
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institutes of Health
- National Heart, Lung, and Blood Institute
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- National Institute of Biomedical Imaging and Bioengineering
- NHLBI Division of Intramural Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- striated muscle, connectomics, lipid droplets, calcium handling, energy distribution, myocardium, oxidative muscle, glycolytic muscle
- MeSH terms
- Animals, Calcium, Energy Metabolism, Male, Mice, Inbred C57BL, Mitochondria, Muscle, Skeletal, Mice, Muscle, Striated, Connectome
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