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Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production

Scrivo, Aurora, Codogno, Patrice, Bomont, Pascale·2019·Nature Communications
DOI 10.1038/s41467-019-08331-wPMC PMC6377711
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Abstract

Autophagy is an essential self-digestion machinery for cell survival and homoeostasis. Membrane elongation is fundamental, as it drives the formation of the double-membrane vesicles that engulf cytosolic material. LC3-lipidation, the signature of autophagosome formation, results from a complex ubiquitin-conjugating cascade orchestrated by the ATG16L1 protein, whose regulation is unknown. Here, we identify the Gigaxonin-E3 ligase as the first regulator of ATG16L1 turn-over and autophagosome production. Gigaxonin interacts with the WD40 domain of ATG16L1 to drive its ubiquitination and subsequent degradation. Gigaxonin depletion induces the formation of ATG16L1 aggregates and impairs LC3 lipidation, hence altering lysosomal fusion and degradation of the main autophagy receptor p62. Altogether, we demonstrate that the Gigaxonin-E3 ligase controls the production of autophagosomes by a reversible, ubiquitin-dependent process selective for ATG16L1. Our findings unveil the fundamental mechanisms of the control of autophagosome formation, and provide a molecular switch to fine-tune the activation of autophagy.

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Metadata

Authors
Scrivo, A., Codogno, P., & Bomont, P.
ORCID
Bomont, Pascale
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2019-02-15
Volume
10
Issue
1
ISSN
2041-1723
DOI
10.1038/s41467-019-08331-w
PMC
PMC6377711
Cited by
64
References
10
Language
en
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