The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
Open accessAbstract
The CRISPR-Cas9 system provides the ability to edit, repress, activate, or mark any gene (or DNA element) by pairing of a programmable single guide RNA (sgRNA) with a complementary sequence on the DNA target. Here we present a new method for small-molecule control of CRISPR-Cas9 function through insertion of RNA aptamers into the sgRNA. We show that CRISPR-Cas9-based gene repression (CRISPRi) can be either activated or deactivated in a dose-dependent fashion over a >10-fold dynamic range in response to two different small-molecule ligands. Since our system acts directly on each target-specific sgRNA, it enables new applications that require differential and opposing temporal control of multiple genes.
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Citations
Cites
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016 · cited 2×
“This strain was originally described in ref.[2].”
BackgroundSelf-citationOptimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Doench, J. G., Fusi, N., Sullender, M., Hegde, M., Vaimberg, E. W., Donovan, K. F., Smith, I., Tothova, Z., Wilen, C., Orchard, R., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3437 · Cited by 4,637
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696
Self-citationCrystal structure of Cas9 in complex with guide RNA and target DNA
Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306
Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
Park, H. M., Liu, H., Wu, J., Chong, A., Mackley, V., Fellmann, C., Rao, A., Jiang, F., Chu, H., Murthy, N., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-05641-3 · Cited by 102
Self-citation
Cited by
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Anzalone, A. V., Koblan, L. W., & Liu, D. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0561-9 · Cited by 2,237
Xiong, Y., Zhang, J., Yang, Z., Mou, Q., Ma, Y., Xiong, Y., & Lu, Y. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.9b09211 · Cited by 689
G-quadruplex-based CRISPR photoswitch for spatiotemporal control of genomic modulation
Deng, H., Xu, H., Wang, Y., Jia, R., Ma, X., Feng, Y., & Chen, H. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad178 · Cited by 45
Genome editing technologies: CRISPR, LEAPER, RESTORE, ARCUT, SATI, and RESCUE
Görücü Yılmaz, Ş. (2021) · IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund · doi:10.17179/excli2020-3070
Metadata
- Authors
- Kundert, K., Lucas, J. E., Watters, K. E., Fellmann, C., Ng, A. H., Heineike, B. M., Fitzsimmons, C. M., Oakes, B. L., Qu, J., Prasad, N., Rosenberg, O. S., Savage, D. F., El-Samad, H., Doudna, J. A., & Kortemme, T.
- Venue
- Nature Communications
- Published
- 2019-05-09
- Volume
- 10
- Issue
- 1
- Pages
- 2127-2127
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2953209503
- Cited by
- 189
- References
- 5
- FWCI
- 8.02
- Type
- journal article
- Language
- en
- Open access
- gold
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- Kundert, K., Lucas, J. E., Watters, K. E., Fellmann, C., Ng, A. H., Heineike, B. M., Fitzsimmons, C. M., Oakes, B. L., Qu, J., Prasad, N., Rosenberg, O. S., Savage, D. F., El-Samad, H., Doudna, J. A., & Kortemme, T. (2019). Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs. Nature Communications. https://doi.org/10.1038/s41467-019-09985-2
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
