The DOI matches its Crossref record; 76 of the 76 references it cites were found in a registry or an index.
Hypoxia-enhanced Blood-Brain Barrier Chip recapitulates human barrier function and shuttling of drugs and antibodies
Open accessAbstract
The high selectivity of the human blood-brain barrier (BBB) restricts delivery of many pharmaceuticals and therapeutic antibodies to the central nervous system. Here, we describe an in vitro microfluidic organ-on-a-chip BBB model lined by induced pluripotent stem cell-derived human brain microvascular endothelium interfaced with primary human brain astrocytes and pericytes that recapitulates the high level of barrier function of the in vivo human BBB for at least one week in culture. The endothelium expresses high levels of tight junction proteins and functional efflux pumps, and it displays selective transcytosis of peptides and antibodies previously observed in vivo. Increased barrier functionality was accomplished using a developmentally-inspired induction protocol that includes a period of differentiation under hypoxic conditions. This enhanced BBB Chip may therefore represent a new in vitro tool for development and validation of delivery systems that transport drugs and therapeutic antibodies across the human BBB.
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Cites
Hypoxia-inducible factors in physiology and medicine
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Self-citationTEER Measurement Techniques for In Vitro Barrier Model Systems
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From 3D cell culture to organs-on-chips
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Self-citationBlood‐brain barrier‐specific properties of a human adult brain endothelial cell line
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Blood-brain barrier active efflux transporters: ATP-binding cassette gene family
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Helms, H. C., Abbott, N. J., Burek, M., Cecchelli, R., Couraud, P.-O., Deli, M. A., Förster, C., Galla, H. J., Romero, I. A., Shusta, E. V., et al. (2016) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x16630991 · Cited by 843
Self-citationSmall airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro
Benam, K. H., Villenave, R., Lucchesi, C., Varone, A., Hubeau, C., Lee, H.-H., Alves, S. E., Salmon, M., Ferrante, T. C., Weaver, J. C., et al. (2016) · Nature Methods · doi:10.1038/nmeth.3697 · Cited by 816
Self-citationDerivation of blood-brain barrier endothelial cells from human pluripotent stem cells
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Self-citationWnt/β-catenin signaling is required for CNS, but not non-CNS, angiogenesis
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Cited by
Human organs-on-chips for disease modelling, drug development and personalized medicine
Ingber, D. E. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-022-00466-9 · Cited by 1,589
Self-citationA guide to the organ-on-a-chip
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Multi-lineage human iPSC-derived platforms for disease modeling and drug discovery
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Human pluripotent stem cell–derived brain pericyte–like cells induce blood-brain barrier properties
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Metadata
- Authors
- Park, T.-E., Mustafaoglu, N., Herland, A., Hasselkus, R., Mannix, R., FitzGerald, E. A., Prantil-Baun, R., Watters, A., Henry, O., Benz, M., Sanchez, H., McCrea, H. J., Goumnerova, L. C., Song, H. W., Palecek, S. P., Shusta, E., & Ingber, D. E.
- Venue
- Nature Communications
- Published
- 2019-06-13
- Volume
- 10
- Issue
- 1
- Pages
- 2621-2621
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2953383090
- Cited by
- 594
- References
- 76
- FWCI
- 27.75
- Type
- journal article
- Language
- en
- Open access
- gold
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- Park, T.-E., Mustafaoglu, N., Herland, A., Hasselkus, R., Mannix, R., FitzGerald, E. A., Prantil-Baun, R., Watters, A., Henry, O., Benz, M., Sanchez, H., McCrea, H. J., Goumnerova, L. C., Song, H. W., Palecek, S. P., Shusta, E., & Ingber, D. E. (2019). Hypoxia-enhanced Blood-Brain Barrier Chip recapitulates human barrier function and shuttling of drugs and antibodies. Nature Communications. https://doi.org/10.1038/s41467-019-10588-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
