Cited by 25Chromatin Remodeling and CancerGenomics and Chromatin DynamicsProtein Structure and Dynamics

Structure of the yeast Swi/Snf complex in a nucleosome free state

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Wang, Chengcheng, Guo, Zhouyan, Zhan, Xiechao, Yang, Fenghua, Wu, Mingxuan, Zhang, Xiaofeng·2020·Nature Communications

Abstract

SWI/SNF remodelers play a key role in regulating chromatin architecture and gene expression. Here, we report the cryo-EM structure of the Saccharomyces cerevisiae Swi/Snf complex in a nucleosome-free state. The structure consists of a stable triangular base module and a flexible Arp module. The conserved subunits Swi1 and Swi3 form the backbone of the complex and closely interact with other components. Snf6, which is specific for yeast Swi/Snf complex, stabilizes the binding of the ATPase-containing subunit Snf2 to the base module. Comparison of the yeast Swi/Snf and RSC complexes reveals conserved structural features that govern the assembly and function of these two subfamilies of chromatin remodelers. Our findings complement those from recent structures of the yeast and human chromatin remodelers and provide further insights into the assembly and function of the SWI/SNF remodelers.

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Authors
Wang, C., Guo, Z., Zhan, X., Yang, F., Wu, M., & Zhang, X.
Affiliations
  • Westlake University (CN)
  • Center for Life Sciences (CN)
Corresponding author
  • Wang, Chengcheng
  • Zhang, Xiaofeng
Venue
Nature Communications
Published
2020-07-07
Volume
11
Issue
1
Pages
3398-3398
ISSN
2041-1723 (ISSN-L)
OpenAlex
W3039567736
Cited by
25
References
50
Citations by year
2026: 5 · 2025: 5 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 3 · 2020: 1
FWCI
1.05
Type
journal article
Language
en
Open access
Locations
  • Nature Communications (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Natural Science Foundation of China (31930059, 81920108015)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
SWI/SNF complex, chromatin remodeling, Saccharomyces cerevisiae, cryo-EM structure, Swi1, RSC complex, complex assembly, chromatin structure
MeSH terms
Protein Domains, Adenosine Triphosphatases, Chromatin, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Humans, Nuclear Proteins, Nucleosomes, Protein Binding, Repressor Proteins, Saccharomyces cerevisiae, Transcription Factors, Cryoelectron Microscopy, Saccharomyces cerevisiae Proteins

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