Structure of the yeast Swi/Snf complex in a nucleosome free state
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SWI/SNF remodelers play a key role in regulating chromatin architecture and gene expression. Here, we report the cryo-EM structure of the Saccharomyces cerevisiae Swi/Snf complex in a nucleosome-free state. The structure consists of a stable triangular base module and a flexible Arp module. The conserved subunits Swi1 and Swi3 form the backbone of the complex and closely interact with other components. Snf6, which is specific for yeast Swi/Snf complex, stabilizes the binding of the ATPase-containing subunit Snf2 to the base module. Comparison of the yeast Swi/Snf and RSC complexes reveals conserved structural features that govern the assembly and function of these two subfamilies of chromatin remodelers. Our findings complement those from recent structures of the yeast and human chromatin remodelers and provide further insights into the assembly and function of the SWI/SNF remodelers.
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The yeast SWI/SNF complex is an ATP-dependent chromatin-remodelling complex composed of 12 subunits, 5 of which are fungal-specific.
“The yeast SWI/SNF complex is an ATP-dependent chromatin-remodelling complex, and it is composed of 12 subunits, 5 of which are fungal-specific36.”
From SWI/SNF and the histone chaperone Rtt106 drive expression of the Pleiotropic Drug Resistance network genes (2022) · cites this paper as [36]
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- Authors
- Wang, C., Guo, Z., Zhan, X., Yang, F., Wu, M., & Zhang, X.
- Affiliations
- Westlake University (CN)
- Center for Life Sciences (CN)
- Corresponding author
- Wang, Chengcheng
- Zhang, Xiaofeng
- Venue
- Nature Communications
- Published
- 2020-07-07
- Volume
- 11
- Issue
- 1
- Pages
- 3398-3398
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC7340788
- OpenAlex
- W3039567736
- Cited by
- 25
- References
- 50
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 3 · 2020: 1
- FWCI
- 1.05
- Type
- journal article
- Language
- en
- Update notices
- correction (2020-11-06)
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (31930059, 81920108015)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- SWI/SNF complex, chromatin remodeling, Saccharomyces cerevisiae, cryo-EM structure, Swi1, RSC complex, complex assembly, chromatin structure
- MeSH terms
- Protein Domains, Adenosine Triphosphatases, Chromatin, Chromosomal Proteins, Non-Histone, DNA-Binding Proteins, Humans, Nuclear Proteins, Nucleosomes, Protein Binding, Repressor Proteins, Saccharomyces cerevisiae, Transcription Factors, Cryoelectron Microscopy, Saccharomyces cerevisiae Proteins
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