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Cited by 37Advanced Electron Microscopy Techniques and ApplicationsBacterial Genetics and BiotechnologyBiochemical and Structural Characterization

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CryoEM structure of the type IVa pilus secretin required for natural competence in Vibrio cholerae

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Weaver, Sara J., Ortega, Davi R., Sazinsky, Matthew H., Dalia, Triana N., Dalia, Ankur B., Jensen, Grant J.·2020·Nature Communications
DOI 10.1038/s41467-020-18866-yPMC PMC7545093

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Abstract

Natural transformation is the process by which bacteria take up genetic material from their environment and integrate it into their genome by homologous recombination. It represents one mode of horizontal gene transfer and contributes to the spread of traits like antibiotic resistance. In Vibrio cholerae , a type IVa pilus (T4aP) is thought to facilitate natural transformation by extending from the cell surface, binding to exogenous DNA, and retracting to thread this DNA through the outer membrane secretin, PilQ. Here, we use a functional tagged allele of VcPilQ purified from native V. cholerae cells to determine the cryoEM structure of the VcPilQ secretin in amphipol to ~2.7 Å. We use bioinformatics to examine the domain architecture and gene neighborhood of T4aP secretins in Proteobacteria in comparison with VcPilQ. This structure highlights differences in the architecture of the T4aP secretin from the type II and type III secretion system secretins. Based on our cryoEM structure, we design a series of mutants to reversibly regulate VcPilQ gate dynamics. These experiments support the idea of VcPilQ as a potential druggable target and provide insight into the channel that DNA likely traverses to promote the spread of antibiotic resistance via horizontal gene transfer by natural transformation.

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Metadata

Authors
Weaver, S. J., Ortega, D. R., Sazinsky, M. H., Dalia, T. N., Dalia, A. B., & Jensen, G. J.
Affiliations
  • California Institute of Technology (US)
  • Pomona College (US)
  • Indiana University Bloomington (US)
Corresponding author
Jensen, Grant J.
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2020-10-08
Volume
11
Issue
1
Pages
5080-5080
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-020-18866-y
PMC
PMC7545093
OpenAlex
W3092186467
Cited by
37
References
1
Citations by year
2026: 7 · 2025: 6 · 2024: 5 · 2023: 7 · 2022: 6 · 2021: 4 · 2020: 2
FWCI
1.94
Type
journal article
Language
en
Update notices
correction (2020-10-27)
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • CaltechAUTHORS (California Institute of Technology) (submittedVersion, cc-by-nc-nd)
  • CaltechAUTHORS (California Institute of Technology) (submittedVersion, cc-by)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
U.S. Department of Health & Human Services | National Institutes of Health (R35GM128674, AI127401)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
Vibrio cholerae, natural competence, natural transformation, horizontal gene transfer, DNA uptake, antibiotic resistance spread, druggable targets, Proteobacteria
MeSH terms
Protein Domains, Cysteine, Membrane Proteins, Models, Molecular, Mutation, Phylogeny, Fimbriae, Bacterial, Secretin, Transformation, Bacterial, Vibrio cholerae, Cryoelectron Microscopy, Bacterial Secretion Systems

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