The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA
Open accessAbstract
The SARS-CoV-2 nucleocapsid (N) protein is an abundant RNA-binding protein critical for viral genome packaging, yet the molecular details that underlie this process are poorly understood. Here we combine single-molecule spectroscopy with all-atom simulations to uncover the molecular details that contribute to N protein function. N protein contains three dynamic disordered regions that house putative transiently-helical binding motifs. The two folded domains interact minimally such that full-length N protein is a flexible and multivalent RNA-binding protein. N protein also undergoes liquid-liquid phase separation when mixed with RNA, and polymer theory predicts that the same multivalent interactions that drive phase separation also engender RNA compaction. We offer a simple symmetry-breaking model that provides a plausible route through which single-genome condensation preferentially occurs over phase separation, suggesting that phase separation offers a convenient macroscopic readout of a key nanoscopic interaction.
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Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor
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Structural basis of receptor recognition by SARS-CoV-2
Shang, J., Ye, G., Shi, K., Wan, Y., Luo, C., Aihara, H., Geng, Q., Auerbach, A., & Li, F. (2020) · Nature · doi:10.1038/s41586-020-2179-y · Cited by 3,661
Developing Covid-19 Vaccines at Pandemic Speed
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G3BP1 is a tunable switch that triggers phase separation to assemble stress granules
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Valence and patterning of aromatic residues determine the phase behavior of prion-like domains
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Self-citationExtreme disorder in an ultrahigh-affinity protein complex
Borgia, A., Borgia, M. B., Bugge, K., Kissling, V. M., Heidarsson, P. O., Fernandes, C. B., Sottini, A., Soranno, A., Buholzer, K. J., Nettels, D., et al. (2018) · Nature · doi:10.1038/nature25762 · Cited by 653
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Cited by
Therapeutic strategies for COVID-19: progress and lessons learned
Li, G., Hilgenfeld, R., Whitley, R., & De Clercq, E. (2023) · Nature Reviews Drug Discovery · doi:10.1038/s41573-023-00672-y · Cited by 676
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Rapid assessment of SARS-CoV-2–evolved variants using virus-like particles
Syed, A. M., Taha, T. Y., Tabata, T., Chen, I. P., Ciling, A., Khalid, M. M., Sreekumar, B., Chen, P.-Y., Hayashi, J. M., Soczek, K. M., et al. (2021) · Science · doi:10.1126/science.abl6184 · Cited by 319
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Metadata
- Authors
- Cubuk, J., Alston, J. J., Incicco, J. J., Singh, S., Stuchell-Brereton, M. D., Ward, M. D., Zimmerman, M. I., Vithani, N., Griffith, D., Wagoner, J. A., Bowman, G. R., Hall, K. B., Soranno, A., & Holehouse, A. S.
- Venue
- Nature Communications
- Published
- 2021-03-29
- Volume
- 12
- Issue
- 1
- Pages
- 1936-1936
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W3143867892
- Cited by
- 576
- References
- 8
- FWCI
- 28.78
- Type
- journal article
- Language
- en
- Open access
- gold
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- Cubuk, J., Alston, J. J., Incicco, J. J., Singh, S., Stuchell-Brereton, M. D., Ward, M. D., Zimmerman, M. I., Vithani, N., Griffith, D., Wagoner, J. A., Bowman, G. R., Hall, K. B., Soranno, A., & Holehouse, A. S. (2021). The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA. Nature Communications. https://doi.org/10.1038/s41467-021-21953-3
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