The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Rapid assessment of SARS-CoV-2–evolved variants using virus-like particles
Open accessAbstract
A tool to probe SARS-CoV-2 biology: To develop therapies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and emerging variants, it is important to understand the viral biology and the effect of mutations. However, this is challenging because live virus can only be studied in a few laboratories that meet stringent safety standards. Syed et al . describe a virus-like particle (VLP) that comprises the four SARS-CoV-2 structural proteins, but instead of packaging viral RNA, it packages messenger RNA (mRNA) that expresses a reporter protein (see the Perspective by Johnson and Menachery). The amount of reporter expressed in receiver cells depends on the efficiency of packaging and assembly in the producer cells and the efficiency of entry into receiver cells. Mutations in the nucleocapsid protein that are found in more transmissible variants increase mRNA packaging and expression. The VLPs provide a platform for studying the effect of mutations in the structural proteins and for screening therapeutics. —VV
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Citations
Cites
SARS-CoV-2 variants, spike mutations and immune escape
Harvey, W. T., Carabelli, A. M., Jackson, B., Gupta, R. K., Thomson, E. C., Harrison, E. M., Ludden, C., Reeve, R., Rambaut, A., COVID-19 Genomics UK (COG-UK) Consortium, et al. (2021) · Nature Reviews Microbiology · doi:10.1038/s41579-021-00573-0 · Cited by 3,528
Spike mutation D614G alters SARS-CoV-2 fitness
Plante, J. A., Liu, Y., Liu, J., Xia, H., Johnson, B. A., Lokugamage, K. G., Zhang, X., Muruato, A. E., Zou, J., Fontes-Garfias, C. R., et al. (2021) · Nature · doi:10.1038/s41586-020-2895-3 · Cited by 1,558 · cited 1×
“evaluation of S-mediated ACE2 receptor binding and cell entry (6, 7).”
Escape from neutralizing antibodies by SARS-CoV-2 spike protein variants
Weisblum, Y., Schmidt, F., Zhang, F., DaSilva, J., Poston, D., Lorenzi, J. C., Muecksch, F., Rutkowska, M., Hoffmann, H.-H., Michailidis, E., et al. (2020) · eLife · doi:10.7554/elife.61312 · Cited by 1,308
Crawford, K. H. D., Eguia, R., Dingens, A. S., Loes, A. N., Malone, K. D., Wolf, C. R., Chu, H. Y., Tortorici, M. A., Veesler, D., Murphy, M., et al. (2020) · Viruses · doi:10.3390/v12050513 · Cited by 776
Transmission, infectivity, and neutralization of a spike L452R SARS-CoV-2 variant
Deng, X., Garcia-Knight, M. A., Khalid, M. M., Servellita, V., Wang, C., Morris, M. K., Sotomayor-González, A., Glasner, D. R., Reyes, K. R., Gliwa, A. S., et al. (2021) · Cell · doi:10.1016/j.cell.2021.04.025 · Cited by 651
Self-citationAn Infectious cDNA Clone of SARS-CoV-2
Xie, X., Muruato, A., Lokugamage, K. G., Narayanan, K., Zhang, X., Zou, J., Liu, J., Schindewolf, C., Bopp, N. E., Aguilar, P. V., et al. (2020) · Cell Host & Microbe · doi:10.1016/j.chom.2020.04.004 · Cited by 647
SARS-CoV-2 spike L452R variant evades cellular immunity and increases infectivity
Motozono, C., Toyoda, M., Zahradnik, J., Saito, A., Nasser, H., Tan, T. S., Ngare, I., Kimura, I., Uriu, K., Kosugi, Y., et al. (2021) · Cell Host & Microbe · doi:10.1016/j.chom.2021.06.006 · Cited by 586
Li, B., Deng, A., Li, K., Hu, Y., Li, Z., Shi, Y., Xiong, Q., Liu, Z., Guo, Q., Zou, L., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-28089-y · Cited by 578
The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA
Cubuk, J., Alston, J. J., Incicco, J. J., Singh, S., Stuchell-Brereton, M. D., Ward, M. D., Zimmerman, M. I., Vithani, N., Griffith, D., Wagoner, J. A., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-21953-3 · Cited by 576
The N501Y spike substitution enhances SARS-CoV-2 transmission
Liu, Y., Liu, J., Plante, K. S., Plante, J. A., Xie, X., Zhang, X., Ku, Z., An, Z., Scharton, D., Schindewolf, C., et al. (2021) · doi:10.1101/2021.03.08.434499 · Cited by 574 · cited 1×
“However, S mutations tested in the context of SARSCoV-2 infectious clones have shown mixed effects, suggesting complex or indirect connections between S and infectivity (24, 25).”
Cited by
Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein
(2022) · doi:10.1093/pnasnexus/pgac049 · Cited by 75
Yang, J., Cui, Y., Yu, D., Zhang, G., Cao, R., Gu, Z., Dai, G., Wu, X., Ling, Y., Yi, C., et al. (2023) · Molecular Biology and Evolution · doi:10.1093/molbev/msad142 · Cited by 15
Human cytokine and coronavirus nucleocapsid protein interactivity using large-scale virtual screens
Tomezsko, P. J., Ford, C. T., Meyer, A. E., Michaleas, A. M., & Jaimes, R. (2024) · Frontiers in Bioinformatics · doi:10.3389/fbinf.2024.1397968 · Cited by 9
Metadata
- Authors
- Syed, A. M., Taha, T. Y., Tabata, T., Chen, I. P., Ciling, A., Khalid, M. M., Sreekumar, B., Chen, P.-Y., Hayashi, J. M., Soczek, K. M., Ott, M., & Doudna, J. A.
- Venue
- Science
- Published
- 2021-12-24
- Volume
- 374
- Issue
- 6575
- Pages
- 1626-1632
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W3210408195
- Cited by
- 319
- References
- 33
- FWCI
- 18.88
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Syed, A. M., Taha, T. Y., Tabata, T., Chen, I. P., Ciling, A., Khalid, M. M., Sreekumar, B., Chen, P.-Y., Hayashi, J. M., Soczek, K. M., Ott, M., & Doudna, J. A. (2021). Rapid assessment of SARS-CoV-2–evolved variants using virus-like particles. Science. https://doi.org/10.1126/science.abl6184
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
