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Cited by 16Bacterial Genetics and BiotechnologyAdvanced Electron Microscopy Techniques and ApplicationsBiochemical and Structural Characterization

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Dual function of OmpM as outer membrane tether and nutrient uptake channel in diderm Firmicutes

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Silale, Augustinas, Zhu, Yiling, Witwinowski, Jerzy, Smith, Robert E., Newman, Kahlan E., Bhamidimarri, Satya P., Baslé, Arnaud, Khalid, Syma, Beloin, Christophe, Gribaldo, Simonetta, van den Berg, Bert·2023·Nature Communications
DOI 10.1038/s41467-023-42601-yPMC PMC10628300

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Abstract

The outer membrane (OM) in diderm, or Gram-negative, bacteria must be tethered to peptidoglycan for mechanical stability and to maintain cell morphology. Most diderm phyla from the Terrabacteria group have recently been shown to lack well-characterised OM attachment systems, but instead have OmpM, which could represent an ancestral tethering system in bacteria. Here, we have determined the structure of the most abundant OmpM protein from Veillonella parvula (diderm Firmicutes) by single particle cryogenic electron microscopy. We also characterised the channel properties of the transmembrane β-barrel of OmpM and investigated the structure and PG-binding properties of its periplasmic stalk region. Our results show that OM tethering and nutrient acquisition are genetically linked in V. parvula , and probably other diderm Terrabacteria. This dual function of OmpM may have played a role in the loss of the OM in ancestral bacteria and the emergence of monoderm bacterial lineages.

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Metadata

Authors
Silale, A., Zhu, Y., Witwinowski, J., Smith, R. E., Newman, K. E., Bhamidimarri, S. P., Baslé, A., Khalid, S., Beloin, C., Gribaldo, S., & van den Berg, B.
Affiliations
  • Newcastle University (GB)
  • Institut Pasteur (FR)
  • University of Southampton (GB)
  • University of Oxford (GB)
Corresponding author
  • Beloin, Christophe
  • Gribaldo, Simonetta
  • van den Berg, Bert
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2023-11-06
Volume
14
Issue
1
Pages
7152-7152
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-023-42601-y
PMC
PMC10628300
OpenAlex
W4388407503
Cited by
16
References
1
Citations by year
2026: 6 · 2025: 6 · 2024: 4
FWCI
2.66
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • ePrints Soton (University of Southampton) (acceptedVersion, cc-by)
  • HAL (submittedVersion, cc-by)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Newcastle University ePrints (Newcastle Univesity) (cc-by)
Funders
  • Wellcome Trust (214222/Z/18/Z)
  • RCUK | Engineering and Physical Sciences Research Council (EP/V030779/1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
Veillonella parvula, Gram-negative bacteria, peptidoglycan binding, transmembrane beta-barrel, bacterial morphology
MeSH terms
Firmicutes, Bacillota, Nutrients, Bacteria, Bacterial Outer Membrane Proteins, Cell Membrane, Cell Wall, Gram-Negative Bacteria

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