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Cited by 67Powdery Mildew Fungal DiseasesWheat and Barley Genetics and PathologyPlant-Microbe Interactions and Immunity

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Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein

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Li, Huanhuan, Men, Wenqiang, Ma, Chao, Liu, Qianwen, Dong, Zhenjie, Tian, Xiubin, Wang, Chaoli, Liu, Cheng, Gill, Harsimardeep S., Ma, Pengtao, Zhang, Zhibin, Liu, Bao, Zhao, Yue, Sehgal, Sunish K., Liu, Wenxuan·2024·Nature Communications
DOI 10.1038/s41467-024-46814-7PMC PMC10951266

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Abstract

Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives of wheat constitute rich sources of diversity for powdery mildew resistance. Here, we report the map-based cloning of the powdery mildew resistance gene Pm13 from the wild wheat species Aegilops longissima . Pm13 encodes a mixed lineage kinase domain-like (MLKL) protein that contains an N-terminal-domain of MLKL (MLKL_NTD) domain in its N-terminus and a C-terminal serine/threonine kinase (STK) domain. The resistance function of Pm13 is validated by mutagenesis, gene silencing, transgenic assay, and allelic association analyses. The development of introgression lines with significantly reduced chromosome segments of Ae. longissima encompassing Pm13 enables widespread deployment of this gene into wheat cultivars. The cloning of Pm13 may provide valuable insights into the molecular mechanisms underlying Pm13 -mediated powdery mildew resistance and highlight the important roles of kinase fusion proteins (KFPs) in wheat immunity.

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Metadata

Authors
Li, H., Men, W., Ma, C., Liu, Q., Dong, Z., Tian, X., Wang, C., Liu, C., Gill, H. S., Ma, P., Zhang, Z., Liu, B., Zhao, Y., Sehgal, S. K., & Liu, W.
Affiliations
  • Henan Agricultural University (CN)
  • Nanjing Agricultural University (CN)
  • Chinese Academy of Sciences (CN)
  • Shandong Academy of Agricultural Sciences (CN)
  • South Dakota State University (US)
  • Yantai University (CN)
  • Northeast Normal University (CN)
Corresponding author
  • Zhao, Yue
  • Sehgal, Sunish K.
  • Liu, Wenxuan
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2024-03-19
Volume
15
Issue
1
Pages
2449-2449
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/s41467-024-46814-7
PMC
PMC10951266
OpenAlex
W4392956091
Cited by
67
References
2
Citations by year
2026: 16 · 2025: 34 · 2024: 14
FWCI
15.62
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (32372089, 31971887, 32272070)
  • the Scientific and Technological Research Project of Henan Province of China
  • the Topnotch Talents of Henan Agricultural University
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Powdery Mildew Fungal Diseases
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
Keywords
wheat powdery mildew, disease resistance genes, MLKL, serine/threonine kinase domain, Aegilops longissima, wild wheat relative, positional cloning, introgression lines, gene silencing, allelic association
MeSH terms
Aegilops, Ascomycota, Plant Diseases, Protein Kinases, Triticum, Genes, Plant, Disease Resistance

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