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Cited by 5Photosynthetic Processes and MechanismsSpectroscopy and Quantum Chemical StudiesMetalloenzymes and iron-sulfur proteins

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Roles of multiple TEF30-associated intermediate complexes in the repair and reassembly of photosystem II in Chlamydomonas reinhardtii

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Wang, Yidi, Wang, Chenxi, Li, Anjie, Liu, Zhenfeng·2025·Nature Plants
DOI 10.1038/s41477-025-02036-3

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Abstract

During oxygenic photosynthesis, photosystem II (PSII) uses light energy for oxidizing water and reducing plastoquinone. It is susceptible to photodamage, and the damaged PSII is repaired through a sophisticated biological process assisted by numerous auxiliary proteins. Here we report the cryogenic electron microscopy structures of four PSII-repair complexes from Chlamydomonas reinhardtii associated with the Thylakoid Enriched Fraction 30 (TEF30, an orthologue of plant MET1) protein-namely, a TEF30-PSII core monomer (TEF30-C), two types of TEF30-PSII core dimers (types I and II, TEF30 2-C 2-I and TEF30 2-C 2-II) and a TEF30-C 2 S-type PSII-LHCII supercomplex (TEF30-C 2 S; S, strongly associated light-harvesting complex II trimer). TEF30 mediates the assembly of CP43 with the RC47 module by clamping on the stromal surfaces and prevents the premature association of peripheral antennae with PSII-C. In the transition from TEF30 2-C 2-I to TEF30 2-C 2-II, TEF30-C 2 S and mature C 2 S 2, one PSII core slides along the dimerization interface against the adjacent one by 22-35 Å, generating a zigzagged surface for accommodating the peripheral antennae. These results suggest that the PSII repair process undergoes multiple TEF30-mediated intermediate states to form intact PSII-LHCII supercomplexes.

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Metadata

Authors
Wang, Y., Wang, C., Li, A., & Liu, Z.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
Liu, Zhenfeng
ORCID
Venue
Nature Plants
Publisher
Springer Science and Business Media LLC
Published
2025-06-27
Volume
11
Issue
7
Pages
1455-1469
ISSN
  • 2055-026X (ISSN-L)
  • 2055-0278
DOI
10.1038/s41477-025-02036-3
OpenAlex
W4411709710
Cited by
5
References
2
Citations by year
2026: 3 · 2025: 2
FWCI
1.50
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Plants (publishedVersion)
  • Research Square (acceptedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (31925024)
  • Chinese Academy of Sciences (YSBR-015 and XDB37020101)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Metalloenzymes and iron-sulfur proteins
Keywords
photosystem II repair, MET1, Chlamydomonas reinhardtii, PSII-LHCII supercomplex, CP43, light-harvesting complex II, photodamage, cryo-EM structure
MeSH terms
Photosynthesis, Plant Proteins, Chlamydomonas reinhardtii, Cryoelectron Microscopy, Thylakoids, Photosystem II Protein Complex, Light-Harvesting Protein Complexes

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Wang, Y., Wang, C., Li, A., & Liu, Z. (2025). Roles of multiple TEF30-associated intermediate complexes in the repair and reassembly of photosystem II in Chlamydomonas reinhardtii. Nature Plants. https://doi.org/10.1038/s41477-025-02036-3