The DOI matches its Crossref record; 45 of the 46 references it cites were found in a registry or an index.
A hybrid erbium(III)–bacteriochlorin near-infrared probe for multiplexed biomedical imaging
Open accessAbstract
Spectrally distinct fluorophores are desired for multiplexed bioimaging. In particular, monitoring biological processes in living mammals needs fluorophores that operate in the 'tissue-transparent' near-infrared (NIR) window, that is, between 700 and 1,700 nm. Here we report a fluorophore system based on molecular erbium(III)-bacteriochlorin complexes with large Stokes shift (>750 nm) and narrowband NIR-to-NIR downconversion spectra (full-width at half-maximum ≤ 32 nm). We have found that the fast (2 × 10 9 s-¹) and near-unity energy transfer from bacteriochlorin triplets to the erbium(III) 4 I 13/2 level overcomes the notorious vibrational overtones quenching, resulting in bright and long-lived (1.73 μs) 1,530 nm luminescence in water. We demonstrate the excitation/emission-multiplexed capability of the complexes in the visualization of dynamic circulatory and metabolic processes in living mice, and through skull tracking of cancer cell metastases in mouse brain. This hybrid probe system facilitates robust multiplexed NIR imaging with high contrast and spatial resolution for applications ranging from fluorescence-guided surgery, diagnostics and intravital microscopy.
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Research works printed but not linked: 1
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Metadata
- Authors
- Wang, T., Wang, S., Liu, Z., He, Z., Yu, P., Zhao, M., Zhang, H., Lu, L., Wang, Z., Wang, Z., Zhang, W., Fan, Y., Sun, C., Zhao, D., Liu, W., Bünzli, J.-C. G., & Zhang, F.
- Affiliations
- Fudan University (CN)
- East China University of Science and Technology (CN)
- ShanghaiTech University (CN)
- École Polytechnique Fédérale de Lausanne (CH)
- Corresponding author
- Wang, Shangfeng
- Zhang, Weian
- Zhang, Fan
- ORCID
- Venue
- Nature Materials
- Published
- 2021-07-29
- Volume
- 20
- Issue
- 11
- Pages
- 1571-1578
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W3186280068
- Cited by
- 308
- References
- 46
- Citations by year
- 2026: 41 · 2025: 71 · 2024: 64 · 2023: 59 · 2022: 58 · 2021: 15
- FWCI
- 14.87
- Type
- journal article
- Language
- en
- Open access
- green
- infoscience.epfl.ch
- Licence
- Locations
- Nature Materials (publishedVersion)
- PubMed (publishedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Funders
- China Postdoctoral Science Foundation (KLH1615200, KLH1615215)
- National Natural Science Foundation of China (22088101, 21725502, 51961145403, 21904023)
- Ministry of Science and Technology of the People’s Republic of China (2017YFA0207303)
- Science and Technology Commission of Shanghai Municipality (20JC1411700, 19490713100, 20490710600, 20S31903700)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Lanthanide and Transition Metal Complexes
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
- Keywords
- erbium(III) complexes, bacteriochlorins, near-infrared fluorescence, Stokes shift, energy transfer, deep tissue imaging, fluorescence-guided surgery, brain metastasis imaging, intravital microscopy
- MeSH terms
- Animals, Erbium, Fluorescent Dyes, Porphyrins, Spectroscopy, Near-Infrared
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