Cited by 148Malaria Research and ControlImmunotherapy and Immune ResponsesNanoparticle-Based Drug Delivery

A malaria vaccine adjuvant based on recombinant antigen binding to liposomes

Open access
Huang, Wei-Chiao, Deng, Bingbing, Lin, Cuiyan, Carter, Kevin A., Geng, Jumin, Razi, Aida, He, Xuedan, Chitgupi, Upendra +9 more·2018·Nature Nanotechnology

Abstract

Pfs25 is a malaria transmission-blocking vaccine antigen candidate, but its apparently limited immunogenicity in humans has hindered clinical development. Here, we show that recombinant, polyhistidine-tagged (his-tagged) Pfs25 can be mixed at the time of immunization with pre-formed liposomes containing cobalt porphyrin-phospholipid, resulting in spontaneous nanoliposome antigen particleization (SNAP). Antigens are stably presented in uniformly orientated display via his-tag insertion in the cobalt porphyrin-phospholipid bilayer, without covalent modification or disruption of antigen conformation. SNAP immunization of mice and rabbits is well tolerated with minimal local reactogenicity, and results in orders-of-magnitude higher functional antibody generation compared with other 'mix-and-inject' adjuvants. Serum-stable antigen binding during transit to draining lymph nodes leads to enhanced antigen uptake by phagocytic antigen-presenting cells, with subsequent generation of long-lived, antigen-specific plasma cells. Seamless multiplexing with four additional his-tagged Plasmodium falciparum polypeptides induces strong and balanced antibody production, illustrating the simplicity of developing multistage particulate vaccines with SNAP immunization.

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  • A recent study used spontaneous nanoliposome antigen particleization to show efficacy of a multistage, multi-antigen vaccine in mice and rabbits.

    “a recent study used spontaneous nanoliposome antigen particleization to show efficacy of a multistage, multi-antigen vaccine in mice and rabbits 116 .”

    From Nanotechnology approaches for global infectious diseases (2021) · cites this paper as [116]

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  1. [27]Shao S Functionalization of cobalt porphyrin–phospholipid bilayers with his-tagged ligands and antigens. Nat. Chem 7, 438– 446 (2015). 25901823 PMC4408904

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Authors
Huang, W.-C., Deng, B., Lin, C., Carter, K. A., Geng, J., Razi, A., He, X., Chitgupi, U., Federizon, J., Sun, B., Long, C. A., Ortega, J., Dutta, S., King, C. R., Miura, K., Lee, S.-M., & Lovell, J. F.
Affiliations
  • University at Buffalo, State University of New York (US)
  • National Institute of Allergy and Infectious Diseases (US)
  • McGill University (CA)
  • Walter Reed Army Institute of Research (US)
Corresponding author
Lovell, Jonathan F.
Venue
Nature Nanotechnology
Published
2018-10-08
Volume
13
Issue
12
Pages
1174-1181
ISSN
  • 1748-3387 (ISSN-L)
  • 1748-3395
OpenAlex
W2894881231
Cited by
148
References
51
Citations by year
2026: 10 · 2025: 18 · 2024: 20 · 2023: 19 · 2022: 20 · 2021: 19 · 2020: 23 · 2019: 17 · 2018: 2
FWCI
14.52
Type
journal article
Language
en
Open access
Locations
  • Nature Nanotechnology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (DP5OD017898)
  • National Institute of Allergy and Infectious Diseases (R21AI122964)
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
Keywords
malaria vaccine, vaccine adjuvants, Pfs25, transmission-blocking vaccine, Plasmodium falciparum, antigen-presenting cells, plasma cells, functional antibodies, particulate vaccines
MeSH terms
RAW 264.7 Cells, Adjuvants, Immunologic, Animals, Antibody Formation, Antigens, Protozoan, Female, Humans, Immunization, Liposomes, Plasmodium falciparum, Rabbits, Recombinant Proteins, Protozoan Proteins, Malaria, Falciparum, Malaria Vaccines, Mice

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