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Synthesis and applications of mirror-image proteins

Harrison, Katriona, Mackay, Angus S., Kambanis, Lucas, Maxwell, Joshua W. C., Payne, Richard J.·2023·Nature Reviews Chemistry

DOI 10.1038/s41570-023-00493-y

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Abstract

The homochirality of biomolecules in nature, such as DNA, RNA, peptides and proteins, has played a critical role in establishing and sustaining life on Earth. This chiral bias has also given synthetic chemists the opportunity to generate molecules with inverted chirality, unlocking valuable new properties and applications. Advances in the field of chemical protein synthesis have underpinned the generation of numerous 'mirror-image' proteins (those comprised entirely of D-amino acids instead of canonical L-amino acids), which cannot be accessed using recombinant expression technologies. This Review seeks to highlight recent work on synthetic mirror-image proteins, with a focus on modern synthetic strategies that have been leveraged to access these complex biomolecules as well as their applications in protein crystallography, drug discovery and the creation of mirror-image life.

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Authors: Harrison, K., Mackay, A. S., Kambanis, L., Maxwell, J. W. C., & Payne, R. J.
Affiliations: The University of Sydney (AU)
Corresponding author: Payne, Richard J.
ORCID: Harrison, Katriona
Mackay, Angus S.
Kambanis, Lucas
Maxwell, Joshua W. C.
Payne, Richard J.
Venue: Nature Reviews Chemistry
Publisher: Springer Science and Business Media LLC
Published: 2023-05-12
Volume: 7
Issue: 6
Pages: 383-404
ISSN: 2397-3358 (ISSN-L)
DOI: 10.1038/s41570-023-00493-y
OpenAlex: W4376254085
Semantic Scholar: 32fb99ce1b8e9791682464fc0e0ddabed11ec5ed
Cited by: 104
References: 174
Citations by year: 2026: 27 · 2025: 33 · 2024: 33 · 2023: 11
FWCI: 2.75
Type: journal article
Language: en
Open access: closed
Licence: www.springernature.com/gp/researchers/text-and-data-mining (tdm)
www.springernature.com/gp/researchers/text-and-data-mining (vor)
Locations: Nature Reviews Chemistry (publishedVersion)
PubMed (publishedVersion)
Topics: Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
Keywords: D-amino acids, homochirality, chemical protein synthesis, protein crystallography, drug discovery, synthetic biology
MeSH terms: Amino Acids, Peptides, Proteins

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Synthesis and applications of mirror-image proteins

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