The DOI matches its Crossref record; 173 of the 174 references it cites were found in a registry or an index.
Synthesis and applications of mirror-image proteins
Abstract
The homochirality of biomolecules in nature, such as DNA, RNA, peptides and proteins, has played a critical role in establishing and sustaining life on Earth. This chiral bias has also given synthetic chemists the opportunity to generate molecules with inverted chirality, unlocking valuable new properties and applications. Advances in the field of chemical protein synthesis have underpinned the generation of numerous 'mirror-image' proteins (those comprised entirely of D-amino acids instead of canonical L-amino acids), which cannot be accessed using recombinant expression technologies. This Review seeks to highlight recent work on synthetic mirror-image proteins, with a focus on modern synthetic strategies that have been leveraged to access these complex biomolecules as well as their applications in protein crystallography, drug discovery and the creation of mirror-image life.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Citations
Cites
Solid Phase Peptide Synthesis. I. The Synthesis of a Tetrapeptide
Merrifield, R. B. (1963) · Journal of the American Chemical Society · doi:10.1021/ja00897a025 · Cited by 7,825
Synthesis of Proteins by Native Chemical Ligation
Dawson, P. E., Muir, T. W., Clark‐Lewis, I., & Kent, S. B. H. (1994) · Science · doi:10.1126/science.7973629 · Cited by 4,190
Glycobiology: Toward Understanding the Function of Sugars
Dwek, R. A. (1996) · Chemical Reviews · doi:10.1021/cr940283b · Cited by 2,800
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Gibson, D. G., Glass, J. I., Lartigue, C., Noskov, V. N., Chuang, R.-Y., Algire, M. A., Benders, G. A., Montague, M. G., Ma, L., Moodie, M. M., et al. (2010) · Science · doi:10.1126/science.1190719 · Cited by 2,638
Therapeutic Peptides: Current Applications and Future Directions
Wang, L., Wang, N., Zhang, W., Cheng, X., Yan, Z., Shao, G., Wang, X., Wang, R., & Fu, C. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00904-4 · Cited by 2,131
Searching for Peptide Ligands with an Epitope Library
Scott, J. K., & Smith, G. P. (1990) · Science · doi:10.1126/science.1696028 · Cited by 2,095
Trends in peptide drug discovery
Muttenthaler, M., King, G. F., Adams, D. J., & Alewood, P. F. (2021) · Nature Reviews Drug Discovery · doi:10.1038/s41573-020-00135-8 · Cited by 2,074
Expressed protein ligation: A general method for protein engineering
Muir, T. W., Sondhi, D., & Cole, P. A. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.12.6705 · Cited by 1,192
A General Method for Site-specific Incorporation of Unnatural Amino Acids into Proteins
Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989) · Science · doi:10.1126/science.2649980 · Cited by 1,019
A brief history of synthetic biology
Cameron, D. E., Bashor, C. J., & Collins, J. J. (2014) · Nature Reviews Microbiology · doi:10.1038/nrmicro3239 · Cited by 988
Research works printed but not linked: 2
- Pasteur, L. Mémoire sur la relation qui peut exister entre la forme cristalline et la composition chimique, et sur la cause de la polarisation rotatoire. C. R. Acad. Sci. 26, 535–538 (1848).
- D’Aloisio, V., Dognini, P., Hutcheon, G. A. & Coxon, C. R. PepTherDia: database and structural composition analysis of approved peptide therapeutics and diagnostics. Drug Discov. 26, 1409–1419 (2021).
Cited by
Sorted by citations
Items 101–104 of 104
Mirror Life and the Case for Adaptive Risk Governance
Gillum, D. R. (2026) · Health Security · doi:10.1177/23265094261480574
Local noise shielding guides the separation of binary chiral mixtures
Zhou, Q., Wang, Y.-R., Wang, P., Huo, J., & Wang, X. (2026) · Physical review. E · doi:10.1103/4c17-6tjh
Xie, R., Zhang, Z., Wei, J., Zhang, F., & Yang, Z. (2026) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2026.141761
Caglioti, C., & Palazzetti, F. (2026) · Symmetry · doi:10.3390/sym18101736
Metadata
- Authors
- Harrison, K., Mackay, A. S., Kambanis, L., Maxwell, J. W. C., & Payne, R. J.
- Affiliations
- The University of Sydney (AU)
- Corresponding author
- Payne, Richard J.
- Venue
- Nature Reviews Chemistry
- Published
- 2023-05-12
- Volume
- 7
- Issue
- 6
- Pages
- 383-404
- ISSN
- 2397-3358 (ISSN-L)
- OpenAlex
- W4376254085
- Semantic Scholar
- 32fb99ce1b8e9791682464fc0e0ddabed11ec5ed
- Cited by
- 104
- References
- 174
- Citations by year
- 2026: 27 · 2025: 33 · 2024: 33 · 2023: 11
- FWCI
- 2.75
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature Reviews Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
- Keywords
- D-amino acids, homochirality, chemical protein synthesis, protein crystallography, drug discovery, synthetic biology
- MeSH terms
- Amino Acids, Peptides, Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
