The DOI matches its Crossref record; 245 of the 245 references it cites were found in a registry or an index.
Human biomimetic liver microphysiology systems in drug development and precision medicine
Open accessAbstract
Microphysiology systems (MPS), also called organs-on-chips and tissue chips, are miniaturized functional units of organs constructed with multiple cell types under a variety of physical and biochemical environmental cues that complement animal models as part of a new paradigm of drug discovery and development. Biomimetic human liver MPS have evolved from simpler 2D cell models, spheroids and organoids to address the increasing need to understand patient-specific mechanisms of complex and rare diseases, the response to therapeutic treatments, and the absorption, distribution, metabolism, excretion and toxicity of potential therapeutics. The parallel development and application of transdisciplinary technologies, including microfluidic devices, bioprinting, engineered matrix materials, defined physiological and pathophysiological media, patient-derived primary cells, and pluripotent stem cells as well as synthetic biology to engineer cell genes and functions, have created the potential to produce patient-specific, biomimetic MPS for detailed mechanistic studies. It is projected that success in the development and maturation of patient-derived MPS with known genotypes and fully matured adult phenotypes will lead to advanced applications in precision medicine. In this Review, we examine human biomimetic liver MPS that are designed to recapitulate the liver acinus structure and functions to enhance our knowledge of the mechanisms of disease progression and of the absorption, distribution, metabolism, excretion and toxicity of therapeutic candidates and drugs as well as to evaluate their mechanisms of action and their application in precision medicine and preclinical trials.
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“The goal of HBL-MPS is to accurately represent the structure and function of the hepatic lobule, including oxygen gradients, zonation, vascularization, and cell signaling (Fig. 10A) [179].”
From Constructing biomimetic microenvironments for liver regeneration (2025) · cites this paper as [179]
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Long, Y., Niu, Y., Liang, K., & Du, Y. (2022) · Trends in Cell Biology · doi:10.1016/j.tcb.2021.10.002 · Cited by 233
Organs-on-chips technologies – A guide from disease models to opportunities for drug development
Monteduro, A. G., Rizzato, S., Caragnano, G., Trapani, A., Giannelli, G., & Maruccio, G. (2023) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2023.115271 · Cited by 193
Liver organ-on-chip models for toxicity studies and risk assessment
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Gerussi, A., Natalini, A., Antonangeli, F., Mancuso, C., Agostinetto, E., Barisani, D., Di Rosa, F., Andrade, R., & Invernizzi, P. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22094557 · Cited by 87
Metadata
- Authors
- Gough, A., Soto-Gutierrez, A., Vernetti, L., Ebrahimkhani, M. R., Stern, A. M., & Taylor, D. L.
- Affiliations
- University of Pittsburgh (US)
- Corresponding author
- Taylor, D. Lansing
- Venue
- Nature Reviews Gastroenterology & Hepatology
- Published
- 2020-12-17
- Volume
- 18
- Issue
- 4
- Pages
- 252-268
- ISSN
- 1759-5045 (ISSN-L)
- 1759-5053
- PMC
- PMC9106093
- OpenAlex
- W3112962917
- Cited by
- 117
- References
- 245
- Citations by year
- 2026: 18 · 2025: 23 · 2024: 21 · 2023: 20 · 2022: 19 · 2021: 16
- FWCI
- 1.15
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Reviews Gastroenterology & Hepatology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (UG3TR003289, U01TR002383)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK117881)
- National Center for Advancing Translational Sciences
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Keywords
- organ-on-a-chip, microphysiological systems, drug development, precision medicine, liver acinus, microfluidics, bioprinting, organoids, spheroids, pluripotent stem cells, synthetic biology, patient-specific disease models
- MeSH terms
- Drug Development, Drug Evaluation, Preclinical, Humans, Liver, Models, Animal, Biomimetics, Microfluidics, Microchip Analytical Procedures, Lab-On-A-Chip Devices, Precision Medicine
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