Developmental origin, functional maintenance and genetic rescue of osteoclasts
Open accessAbstract
Osteoclasts are multinucleated giant cells that resorb bone, ensuring development and continuous remodelling of the skeleton and the bone marrow haematopoietic niche. Defective osteoclast activity leads to osteopetrosis and bone marrow failure 1-9, whereas excess activity can contribute to bone loss and osteoporosis 10. Osteopetrosis can be partially treated by bone marrow transplantation in humans and mice 11-18, consistent with a haematopoietic origin of osteoclasts 13,16,19 and studies that suggest that they develop by fusion of monocytic precursors derived from haematopoietic stem cells in the presence of CSF1 and RANK ligand 1,20. However, the developmental origin and lifespan of osteoclasts, and the mechanisms that ensure maintenance of osteoclast function throughout life in vivo remain largely unexplored. Here we report that osteoclasts that colonize fetal ossification centres originate from embryonic erythro-myeloid progenitors 21,22. These erythro-myeloid progenitor-derived osteoclasts are required for normal bone development and tooth eruption. Yet, timely transfusion of haematopoietic-stem-cell-derived monocytic cells in newborn mice is sufficient to rescue bone development in early-onset autosomal recessive osteopetrosis. We also found that the postnatal maintenance of osteoclasts, bone mass and the bone marrow cavity involve iterative fusion of circulating blood monocytic cells with long-lived osteoclast syncytia. As a consequence, parabiosis or transfusion of monocytic cells results in long-term gene transfer in osteoclasts in the absence of haematopoietic-stem-cell chimerism, and can rescue an adult-onset osteopetrotic phenotype caused by cathepsin K deficiency 23,24. In sum, our results identify the developmental origin of osteoclasts and a mechanism that controls their maintenance in bones after birth. These data suggest strategies to rescue osteoclast deficiency in osteopetrosis and to modulate osteoclast activity in vivo.
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Osteoclasts can survive longer by fusing with circulating monocytes that have access to BRC.
“However, the observation that osteoclasts can survive longer by fusing with circulating monocytes with access to BRC255 has challenged this view.”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [255]
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- Authors
- Jacome-Galarza, C. E., Percin, G. I., Muller, J. T., Mass, E., Lazarov, T., Eitler, J., Rauner, M., Yadav, V. K., Crozet, L., Bohm, M., Loyher, P.-L., Karsenty, G., Waskow, C., & Geissmann, F.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Technische Universität Dresden (DE)
- Columbia University Irving Medical Center (US)
- Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) (DE)
- Corresponding author
- Waskow, Claudia
- ORCID
- Venue
- Nature
- Published
- 2019-04
- Volume
- 568
- Issue
- 7753
- Pages
- 541-545
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC6910203
- OpenAlex
- W2936121316
- Cited by
- 475
- References
- 46
- Citations by year
- 2026: 33 · 2025: 59 · 2024: 83 · 2023: 70 · 2022: 76 · 2021: 74 · 2020: 61 · 2019: 19
- FWCI
- 19.18
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- National Institute on Aging (P01AG032959)
- National Heart, Lung, and Blood Institute (R01HL138090)
- National Cancer Institute (T32CA009149, P30CA008748)
- National Institute of Allergy and Infectious Diseases (R01AI130345)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Rheumatology › Osteomyelitis and Bone Disorders Research
- Health Sciences › Medicine › Hematology › Platelet Disorders and Treatments
- Keywords
- osteoclasts, osteoclast origin, osteopetrosis, erythro-myeloid progenitors, skeletal development, tooth eruption, bone marrow microenvironment, hematopoietic stem cells, CSF-1, RANK ligand, parabiosis, bone remodeling
- MeSH terms
- Animals, Animals, Newborn, Bone Development, Female, Genes, Recessive, Hematopoietic Stem Cells, Male, Osteoclasts, Osteopetrosis, Tooth Eruption, Mice
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