The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Controlled modelling of human epiblast and amnion development using stem cells
Open accessAbstract
Early human embryonic development involves extensive lineage diversification, cell-fate specification and tissue patterning1. Despite its basic and clinical importance, early human embryonic development remains relatively unexplained owing to interspecies divergence2,3 and limited accessibility to human embryo samples. Here we report that human pluripotent stem cells (hPSCs) in a microfluidic device recapitulate, in a highly controllable and scalable fashion, landmarks of the development of the epiblast and amniotic ectoderm parts of the conceptus, including lumenogenesis of the epiblast and the resultant pro-amniotic cavity, formation of a bipolar embryonic sac, and specification of primordial germ cells and primitive streak cells. We further show that amniotic ectoderm-like cells function as a signalling centre to trigger the onset of gastrulation-like events in hPSCs. Given its controllability and scalability, the microfluidic model provides a powerful experimental system to advance knowledge of human embryology and reproduction. This model could assist in the rational design of differentiation protocols of hPSCs for disease modelling and cell therapy, and in high-throughput drug and toxicity screens to prevent pregnancy failure and birth defects. Landmarks of early stages of human embryogenesis can be recapitulated in a highly controllable and scalable fashion by culturing human pluripotent stem cells in a microfluidic device.
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Cites
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
Integrating single-cell transcriptomic data across different conditions, technologies, and species
Butler, A., Hoffman, P., Smibert, P., Papalexi, E., & Satija, R. (2018) · Nature Biotechnology · doi:10.1038/nbt.4096 · Cited by 13,174
Spatial reconstruction of single-cell gene expression data
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A ROCK inhibitor permits survival of dissociated human embryonic stem cells
Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J. B., Nishikawa, S., Nishikawa, S.-I., Muguruma, K., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1310 · Cited by 2,055
A method to recapitulate early embryonic spatial patterning in human embryonic stem cells
Warmflash, A., Sorre, B., Etoc, F., Siggia, E. D., & Brivanlou, A. H. (2014) · Nature Methods · doi:10.1038/nmeth.3016 · Cited by 885
SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate
Irie, N., Weinberger, L., Tang, W. W. C., Kobayashi, T., Viukov, S., Manor, Y. S., Dietmann, S., Hanna, J. H., & Surani, M. A. (2015) · Cell · doi:10.1016/j.cell.2014.12.013 · Cited by 839
Self-organization of the human embryo in the absence of maternal tissues
Shahbazi, M. N., Jedrusik, A., Vuoristo, S., Recher, G., Hupalowska, A., Bolton, V., Fogarty, N. M. E., Campbell, A., Devito, L. G., Ilic, D., et al. (2016) · Nature Cell Biology · doi:10.1038/ncb3347 · Cited by 688
Self-organization of the in vitro attached human embryo
Deglincerti, A., Croft, G. F., Pietila, L. N., Zernicka-Goetz, M., Siggia, E. D., & Brivanlou, A. H. (2016) · Nature · doi:10.1038/nature17948 · Cited by 680
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Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells
Sasaki, K., Yokobayashi, S., Nakamura, T., Okamoto, I., Yabuta, Y., Kurimoto, K., Ohta, H., Moritoki, Y., Iwatani, C., Tsuchiya, H., et al. (2015) · Cell Stem Cell · doi:10.1016/j.stem.2015.06.014 · Cited by 551
Cited by
Induced pluripotent stem cells (iPSCs): molecular mechanisms of induction and applications
Cerneckis, J., Cai, H., & Shi, Y. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01809-0 · Cited by 452
Naive stem cell blastocyst model captures human embryo lineage segregation
Yanagida, A., Spindlow, D., Nichols, J., Dattani, A., Smith, A., & Guo, G. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2021.04.031 · Cited by 320
Capturing Cardiogenesis in Gastruloids
Rossi, G., Broguiere, N., Miyamoto, M., Boni, A., Guiet, R., Girgin, M., Kelly, R. G., Kwon, C., & Lutolf, M. P. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2020.10.013 · Cited by 306
Microfluidic high-throughput 3D cell culture
Ko, J., Park, D., Lee, J., Jung, S., Baek, K., Sung, K. E., Lee, J., & Jeon, N. L. (2024) · Nature Reviews Bioengineering · doi:10.1038/s44222-024-00163-8 · Cited by 88
Nicheformer: a foundation model for single-cell and spatial omics
Tejada-Lapuerta, A., Schaar, A. C., Gutgesell, R., Palla, G., Halle, L., Minaeva, M., Vornholz, L., Dony, L., Drummer, F., Richter, T., et al. (2025) · Nature Methods · doi:10.1038/s41592-025-02814-z · Cited by 100
Metadata
- Authors
- Zheng, Y., Xue, X., Shao, Y., Wang, S., Esfahani, S. N., Li, Z., Muncie, J. M., Lakins, J. N., Weaver, V. M., Gumucio, D. L., & Fu, J.
- Venue
- Nature
- Published
- 2019-09-11
- Volume
- 573
- Issue
- 7774
- Pages
- 421-425
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2972524623
- Cited by
- 454
- References
- 35
- FWCI
- 16.47
- Type
- journal article
- Language
- en
- Open access
- green
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- Zheng, Y., Xue, X., Shao, Y., Wang, S., Esfahani, S. N., Li, Z., Muncie, J. M., Lakins, J. N., Weaver, V. M., Gumucio, D. L., & Fu, J. (2019). Controlled modelling of human epiblast and amnion development using stem cells. Nature. https://doi.org/10.1038/s41586-019-1535-2
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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