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Characterizing expression changes in noncoding RNAs during aging and heterochronic parabiosis across mouse tissues

Wagner, Viktoria, Kern, Fabian, Hahn, Oliver, Schaum, Nicholas, Ludwig, Nicole, Fehlmann, Tobias, Engel, Annika, Henn, Dominic +10 more·2024·Nature Biotechnology
DOI 10.1038/s41587-023-01751-6PMC PMC10791587
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Abstract

Molecular mechanisms of organismal and cell aging remain incompletely understood. We, therefore, generated a body-wide map of noncoding RNA (ncRNA) expression in aging (16 organs at ten timepoints from 1 to 27 months) and rejuvenated mice. We found molecular aging trajectories are largely tissue-specific except for eight broadly deregulated microRNAs (miRNAs). Their individual abundance mirrors their presence in circulating plasma and extracellular vesicles (EVs) whereas tissue-specific ncRNAs were less present. For miR-29c-3p, we observe the largest correlation with aging in solid organs, plasma and EVs. In mice rejuvenated by heterochronic parabiosis, miR-29c-3p was the most prominent miRNA restored to similar levels found in young liver. miR-29c-3p targets the extracellular matrix and secretion pathways, known to be implicated in aging. We provide a map of organism-wide expression of ncRNAs with aging and rejuvenation and identify a set of broadly deregulated miRNAs, which may function as systemic regulators of aging via plasma and EVs.

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Sorted by citations

Authors
Wagner, V., Kern, F., Hahn, O., Schaum, N., Ludwig, N., Fehlmann, T., Engel, A., Henn, D., Rishik, S., Isakova, A., Tan, M., Sit, R., Neff, N., Hart, M., Meese, E., Quake, S., Wyss-Coray, T., & Keller, A.
Venue
Nature Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2023-04-27
Volume
42
Issue
1
Pages
109-118
ISSN
  • 1087-0156
  • 1546-1696
DOI
10.1038/s41587-023-01751-6
PMC
PMC10791587
Cited by
71
References
6
Language
en
Licence
Funders
  • Michael J. Fox Foundation for Parkinson’s Research (MJFF-021418)
  • Schaller-Nikolich Foundation

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