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Slow release of a synthetic auxin induces formation of adventitious roots in recalcitrant woody plants

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Roth, Ohad, Yechezkel, Sela, Serero, Ori, Eliyahu, Avi, Vints, Inna, Tzeela, Pan, Carignano, Alberto, Janacek, Dorina P., Peters, Verena, Kessel, Amit, Dwivedi, Vikas, Carmeli-Weissberg, Mira, Shaya, Felix, Faigenboim-Doron, Adi, Ung, Kien Lam, Pedersen, Bjørn Panyella, Riov, Joseph, Klavins, Eric, Dawid, Corinna, Hammes, Ulrich Z., Ben-Tal, Nir, Napier, Richard, Sadot, Einat, Weinstain, Roy·2024·Nature Biotechnology
DOI 10.1038/s41587-023-02065-3

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Abstract

Clonal propagation of plants by induction of adventitious roots (ARs) from stem cuttings is a requisite step in breeding programs. A major barrier exists for propagating valuable plants that naturally have low capacity to form ARs. Due to the central role of auxin in organogenesis, indole-3-butyric acid is often used as part of commercial rooting mixtures, yet many recalcitrant plants do not form ARs in response to this treatment. Here we describe the synthesis and screening of a focused library of synthetic auxin conjugates in Eucalyptus grandis cuttings and identify 4-chlorophenoxyacetic acid-L-tryptophan-OMe as a competent enhancer of adventitious rooting in a number of recalcitrant woody plants, including apple and argan. Comprehensive metabolic and functional analyses reveal that this activity is engendered by prolonged auxin signaling due to initial fast uptake and slow release and clearance of the free auxin 4-chlorophenoxyacetic acid. This work highlights the utility of a slow-release strategy for bioactive compounds for more effective plant growth regulation.

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Metadata

Authors
Roth, O., Yechezkel, S., Serero, O., Eliyahu, A., Vints, I., Tzeela, P., Carignano, A., Janacek, D. P., Peters, V., Kessel, A., Dwivedi, V., Carmeli-Weissberg, M., Shaya, F., Faigenboim-Doron, A., Ung, K. L., Pedersen, B. P., Riov, J., Klavins, E., Dawid, C., Hammes, U. Z., Ben-Tal, N., Napier, R., Sadot, E., & Weinstain, R.
Affiliations
  • Tel Aviv University (IL)
  • Agricultural Research Organization (IL)
  • Hebrew University of Jerusalem (IL)
  • University of Washington (US)
  • Technical University of Munich (DE)
  • Aarhus University (DK)
  • University of Warwick (GB)
Corresponding author
  • Sadot, Einat
  • Weinstain, Roy
ORCID
Venue
Nature Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2024-01-24
Volume
42
Issue
11
Pages
1705-1716
ISSN
  • 0733-222X (ISSN-L)
  • 1087-0156
  • 1546-1696
DOI
10.1038/s41587-023-02065-3
OpenAlex
W4391169111
Cited by
39
References
4
Citations by year
2026: 15 · 2025: 11 · 2024: 13
FWCI
9.79
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Biotechnology (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Warwick Research Archive Portal (University of Warwick) (acceptedVersion)
  • mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion)
Funders
  • Israel Science Foundation (1057/21)
  • United States - Israel Binational Agricultural Research and Development Fund (IS-5195-19R)
  • the Chief Scientist of the Ministry of Agriculture and Rural Development, Israel
  • Deutsche Forschungsgemeinschaft (HA3468/6-3)
  • Tel Aviv University Center for AI and Data Science
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
Keywords
adventitious rooting, slow release, 4-chlorophenoxyacetic acid, indole-3-butyric acid, Eucalyptus grandis, stem cuttings, clonal propagation, auxin signaling, apple, argan, plant growth regulators
MeSH terms
Eucalyptus, Indoleacetic Acids, Plant Growth Regulators, Wood, Plant Roots

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