The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Memory CD4+ T cells are generated in the human fetal intestine
Open accessAbstract
The fetus is thought to be protected from exposure to foreign antigens, yet CD45RO+ T cells reside in the fetal intestine. Here we combined functional assays with mass cytometry, single-cell RNA sequencing and high-throughput T cell antigen receptor (TCR) sequencing to characterize the CD4+ T cell compartment in the human fetal intestine. We identified 22 CD4+ T cell clusters, including naive-like, regulatory-like and memory-like subpopulations, which were confirmed and further characterized at the transcriptional level. Memory-like CD4+ T cells had high expression of Ki-67, indicative of cell division, and CD5, a surrogate marker of TCR avidity, and produced the cytokines IFN-γ and IL-2. Pathway analysis revealed a differentiation trajectory associated with cellular activation and proinflammatory effector functions, and TCR repertoire analysis indicated clonal expansions, distinct repertoire characteristics and interconnections between subpopulations of memory-like CD4+ T cells. Imaging mass cytometry indicated that memory-like CD4+ T cells colocalized with antigen-presenting cells. Collectively, these results provide evidence for the generation of memory-like CD4+ T cells in the human fetal intestine that is consistent with exposure to foreign antigens. Koning and colleagues used mass cytometry, single-cell RNA-seq and high-throughput TCR sequencing to characterize the CD4+ T cell compartment in the human fetal intestine.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Integrating single-cell transcriptomic data across different conditions, technologies, and species
Butler, A., Hoffman, P., Smibert, P., Papalexi, E., & Satija, R. (2018) · Nature Biotechnology · doi:10.1038/nbt.4096 · Cited by 13,174
Massively parallel digital transcriptional profiling of single cells
Zheng, G. X. Y., Terry, J. M., Belgrader, P., Ryvkin, P., Bent, Z. W., Wilson, R., Ziraldo, S. B., Wheeler, T. D., McDermott, G. P., Zhu, J., et al. (2017) · Nature Communications · doi:10.1038/ncomms14049 · Cited by 7,353
Trapnell, C., Cacchiarelli, D., Grimsby, J., Pokharel, P., Li, S., Morse, M., Lennon, N. J., Livak, K. J., Mikkelsen, T. S., & Rinn, J. L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2859 · Cited by 7,314
The maternal microbiota drives early postnatal innate immune development
Gomez de Agüero, M., Ganal-Vonarburg, S. C., Fuhrer, T., Rupp, S., Uchimura, Y., Li, H., Steinert, A., Heikenwalder, M., Hapfelmeier, S., Sauer, U., et al. (2016) · Science · doi:10.1126/science.aad2571 · Cited by 1,231
Kimmig, S., Przybylski, G. K., Schmidt, C. A., Laurisch, K., Möwes, B., Radbruch, A., & Thiel, A. (2002) · The Journal of Experimental Medicine · doi:10.1084/jem.20011756 · Cited by 439
Thome, J. J. C., Bickham, K. L., Ohmura, Y., Kubota, M., Matsuoka, N., Gordon, C., Granot, T., Griesemer, A., Lerner, H., Kato, T., et al. (2016) · Nature Medicine · doi:10.1038/nm.4008 · Cited by 305
A mechanism for expansion of regulatory T-cell repertoire and its role in self-tolerance
Feng, Y., van der Veeken, J., Shugay, M., Putintseva, E. V., Osmanbeyoglu, H. U., Dikiy, S., Hoyos, B. E., Moltedo, B., Hemmers, S., Treuting, P., et al. (2015) · Nature · doi:10.1038/nature16141 · Cited by 157
Self-citation
Cited by
Cells of the human intestinal tract mapped across space and time
Elmentaite, R., Kumasaka, N., Roberts, K., Fleming, A., Dann, E., King, H. W., Kleshchevnikov, V., Dabrowska, M., Pritchard, S., Bolt, L., et al. (2021) · Nature · doi:10.1038/s41586-021-03852-1 · Cited by 699
Mapping the developing human immune system across organs
Suo, C., Dann, E., Goh, I., Jardine, L., Kleshchevnikov, V., Park, J.-E., Botting, R. A., Stephenson, E., Engelbert, J., Tuong, Z. K., et al. (2022) · Science · doi:10.1126/science.abo0510 · Cited by 340
Cano-Gamez, K., Soskic, B., Roumeliotis, T. I., So, E., Smyth, D. J., Baldrighi, M., Willé, D., Nakic, N., Esparza-Gordillo, J., Larminie, C. G. C., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-15543-y · Cited by 274
Elmentaite, R., Ross, A. D. B., Roberts, K., James, K. R., Ortmann, D., Gomes, T., Nayak, K., Tuck, L., Pritchard, S., Bayraktar, O. A., et al. (2020) · Developmental Cell · doi:10.1016/j.devcel.2020.11.010 · Cited by 316
Viable bacterial colonization is highly limited in the human intestine in utero
Rackaityte, E., Halkias, J., Fukui, E. M., Mendoza, V. F., Hayzelden, C., Crawford, E. D., Fujimura, K. E., Burt, T. D., & Lynch, S. V. (2020) · Nature Medicine · doi:10.1038/s41591-020-0761-3 · Cited by 300
Metadata
- Authors
- Li, N., van Unen, V., Abdelaal, T., Guo, N., Kasatskaya, S. A., Ladell, K., McLaren, J. E., Egorov, E. S., Izraelson, M., Chuva de Sousa Lopes, S. M., Höllt, T., Britanova, O. V., Eggermont, J., de Miranda, N. F. C. C., Chudakov, D. M., Price, D. A., Lelieveldt, B. P. F., & Koning, F.
- Venue
- Nature Immunology
- Published
- 2019-01-21
- Volume
- 20
- Issue
- 3
- Pages
- 301-312
- ISSN
- 1529-2908 (ISSN-L)
- 1529-2916
- OpenAlex
- W2909781249
- Cited by
- 168
- References
- 7
- FWCI
- 8.33
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Memory CD4+ T cells are generated in the human fetal intestine. https://app.cochat.ai/papers/10.1038/s41590-018-0294-9
- The paper
- Li, N., van Unen, V., Abdelaal, T., Guo, N., Kasatskaya, S. A., Ladell, K., McLaren, J. E., Egorov, E. S., Izraelson, M., Chuva de Sousa Lopes, S. M., Höllt, T., Britanova, O. V., Eggermont, J., de Miranda, N. F. C. C., Chudakov, D. M., Price, D. A., Lelieveldt, B. P. F., & Koning, F. (2019). Memory CD4+ T cells are generated in the human fetal intestine. Nature Immunology. https://doi.org/10.1038/s41590-018-0294-9
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
