Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood
Open accessAbstract
During ageing thymic function declines and is unable to meet the demand for peripheral T helper (Th) cell replenishment. Therefore, population maintenance of naive Th cells must be at least partly peripherally based. Such peripheral postthymic expansion of recent thymic emigrants (RTEs) during ageing consequently should lead to loss or dilution of T cell receptor excision circles (TRECs) from a subset of naive T cells. We have identified two subsets of naive Th cells in human adult peripheral blood characterized by a striking unequal content of TRECs, indicating different peripheral proliferative histories. TRECs are highly enriched in peripheral naive CD45RA+ Th cells coexpressing CD31 compared with peripheral naive CD45RA+ Th cells lacking CD31 expression, in which TRECs can hardly be detected. Furthermore we show that CD31−CD45RA+ Th cells account for increasing percentages of the naive peripheral Th cell pool during ageing but retain phenotypic and functional features of naive Th cells. As CD31 is lost upon T cell receptor (TCR) engagement in vitro, we hypothesize that TCR triggering is a prerequisite for homeostatically driven peripheral postthymic expansion of human naive RTEs. We describe here the identification of peripherally expanded naive Th cells in human adult blood characterized by the loss of CD31 expression and a highly reduced TREC content.
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Protein tyrosine kinase 7 and CD31 are expressed on a subpopulation of naive cells in children and young adults, are lost with homeostatic proliferation, and decline with age.
“The cell surface molecules protein tyrosine kinase 7 and CD31, expressed on a subpopulation of naive cells in children and young adults, are lost with homeostatic proliferation and decline with age”
From Successful and Maladaptive T Cell Aging (2017) · cites this paper as Kimmig et al., 2002
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- Authors
- Kimmig, S., Przybylski, G. K., Schmidt, C. A., Laurisch, K., Möwes, B., Radbruch, A., & Thiel, A.
- Affiliations
- German Rheumatism Research Centre (DE)
- Humboldt-Universität zu Berlin (DE)
- 1Department of Clinical Immunology, Deutsches Rheuma-Forschungszentrum Berlin, 10117 Berlin, Germany
- Corresponding author
- Thiel, Andreas
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 2002-03-18
- Volume
- 195
- Issue
- 6
- Pages
- 789-794
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- PMC
- PMC2193736
- OpenAlex
- W2059239713
- Cited by
- 439
- References
- 30
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 9 · 2023: 6 · 2022: 4 · 2021: 13 · 2020: 17 · 2019: 27 · 2018: 22 · 2017: 21 · 2016: 21 · 2015: 17 · 2014: 24 · 2013: 34 · 2012: 33
- FWCI
- 4.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Experimental Medicine (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- TREC, CD31, CD45RA, recent thymic emigrants, T cell receptor engagement, T cell proliferation, peripheral blood T cells
- MeSH terms
- Adult, Aging, Flow Cytometry, Humans, Receptors, Antigen, T-Cell, Thymus Gland, T-Lymphocyte Subsets, Leukocyte Common Antigens, Antigens, CD34
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