Cited by 276CRISPR and Genetic EngineeringRNA regulation and diseaseAnimal Genetics and Reproduction

In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice

Zhou, Haibo, Liu, Junlai, Zhou, Changyang, Gao, Ni, Rao, Zhiping, Li, He, Hu, Xinde, Li, Changlin +14 more·2018·Nature Neuroscience

Abstract

Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system. As proof of concept, we were able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into functional neurons in vivo. This system provides a flexible and rapid screening platform for studying complex gene networks and gain-of-function phenotypes in the mammalian brain.

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Crossref · 10.1038/s41593-017-0060-6

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Authors
Zhou, H., Liu, J., Zhou, C., Gao, N., Rao, Z., Li, H., Hu, X., Li, C., Yao, X., Shen, X., Sun, Y., Wei, Y., Liu, F., Ying, W., Zhang, J., Tang, C., Zhang, X., Xu, H., Shi, L., Cheng, L., Huang, P., & Yang, H.
Affiliations
  • Chinese Academy of Sciences (CN)
  • ShanghaiTech University (CN)
Corresponding author
  • Huang, Pengyu
  • Yang, Hui
Venue
Nature Neuroscience
Published
2018-01-15
Volume
21
Issue
3
Pages
440-446
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
OpenAlex
W2783322400
Cited by
276
References
57
Citations by year
2026: 10 · 2025: 26 · 2024: 37 · 2023: 36 · 2022: 38 · 2021: 35 · 2020: 39 · 2019: 34 · 2018: 20
FWCI
11.38
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
Keywords
CRISPR/dCas9, transcriptional activation, transgenic mice, long non-coding RNA, astrocyte-to-neuron conversion, neurogenesis, gene networks, brain
MeSH terms
Animals, Astrocytes, Brain Chemistry, DNA-Binding Proteins, Female, Male, Mice, Transgenic, Nerve Tissue Proteins, Nervous System, Neurons, Nuclear Proteins, Transcriptional Activation, Mice, Primary Cell Culture, RNA, Long Noncoding, CRISPR-Cas Systems

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