The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice
Abstract
Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system. As proof of concept, we were able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into functional neurons in vivo. This system provides a flexible and rapid screening platform for studying complex gene networks and gain-of-function phenotypes in the mammalian brain.
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“a mechanism which is known to regulate LD concentrations27–29”
From APOE4/4 is linked to damaging lipid droplets in Alzheimer’s disease microglia (2024) · cites this paper as [29]
Citations
Cites
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CRISPR/Cas9 therapeutics: progress and prospects
Li, T., Yang, Y., Qi, H., Cui, W., Zhang, L., Fu, X., He, X., Liu, M., Li, P., & Yu, T. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01309-7 · Cited by 824
APOE4/4 is linked to damaging lipid droplets in Alzheimer’s disease microglia
Haney, M. S., Pálovics, R., Munson, C. N., Long, C., Johansson, P. K., Yip, O., Dong, W., Rawat, E., West, E., Schlachetzki, J. C. M., et al. (2024) · Nature · doi:10.1038/s41586-024-07185-7 · Cited by 536
“…indicate that GNE-317 treatment reduced LD concentrations through increasing autophagy, a mechanism which is known to regulate LD concentrations 27–29 ; however, these observations do not conclusively show increased autophagic flux after GNE-317 treatment. were then grown in complete media…”
Engineering the next generation of cell-based therapeutics
Bashor, C. J., Hilton, I. B., Bandukwala, H. S., Smith, D. M., & Omid Veiseh, A. (2022) · Nature Reviews Drug Discovery · doi:10.1038/s41573-022-00476-6 · Cited by 472
Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice
Zhou, H., Su, J., Hu, X., Zhou, C., Li, H., Chen, Z., Xiao, Q., Wang, B., Wu, W., Sun, Y., et al. (2020) · Cell · doi:10.1016/j.cell.2020.03.024 · Cited by 437
Self-citationTissue Engineering and Regenerative Medicine: Achievements, Future, and Sustainability in Asia
Han, F., Wang, J., Ding, L., Hu, Y., Li, W., Yuan, Z., Guo, Q., Zhu, C., Yu, L., Wang, H., et al. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00083 · Cited by 382
Metadata
- Authors
- Zhou, H., Liu, J., Zhou, C., Gao, N., Rao, Z., Li, H., Hu, X., Li, C., Yao, X., Shen, X., Sun, Y., Wei, Y., Liu, F., Ying, W., Zhang, J., Tang, C., Zhang, X., Xu, H., Shi, L., Cheng, L., Huang, P., & Yang, H.
- Affiliations
- Chinese Academy of Sciences (CN)
- ShanghaiTech University (CN)
- Corresponding author
- Huang, Pengyu
- Yang, Hui
- ORCID
- Venue
- Nature Neuroscience
- Published
- 2018-01-15
- Volume
- 21
- Issue
- 3
- Pages
- 440-446
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- OpenAlex
- W2783322400
- Cited by
- 276
- References
- 57
- Citations by year
- 2026: 10 · 2025: 26 · 2024: 37 · 2023: 36 · 2022: 38 · 2021: 35 · 2020: 39 · 2019: 34 · 2018: 20
- FWCI
- 11.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- CRISPR/dCas9, transcriptional activation, transgenic mice, long non-coding RNA, astrocyte-to-neuron conversion, neurogenesis, gene networks, brain
- MeSH terms
- Animals, Astrocytes, Brain Chemistry, DNA-Binding Proteins, Female, Male, Mice, Transgenic, Nerve Tissue Proteins, Nervous System, Neurons, Nuclear Proteins, Transcriptional Activation, Mice, Primary Cell Culture, RNA, Long Noncoding, CRISPR-Cas Systems
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