Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings
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Abstract
The nuclear envelope has to be reformed after mitosis to create viable daughter cells with closed nuclei. How membrane sealing of DNA and assembly of nuclear pore complexes (NPCs) are achieved and coordinated is poorly understood. Here, we reconstructed nuclear membrane topology and the structures of assembling NPCs in a correlative 3D EM time course of dividing human cells. Our quantitative ultrastructural analysis shows that nuclear membranes form from highly fenestrated ER sheets whose holes progressively shrink. NPC precursors are found in small membrane holes and dilate radially during assembly of the inner ring complex, forming thousands of transport channels within minutes. This mechanism is fundamentally different from that of interphase NPC assembly and explains how mitotic cells can rapidly establish a closed nuclear compartment while making it transport competent.
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Some species maintain the fusion topology in the absence of NPCs, e.g. during semi-closed mitosis in Drosophila melanogaster.
“Indeed, some species maintain the fusion topology in the absence of NPCs, e.g. during semi-closed mitosis in Drosophila melanogaster (59).”
From AI-based structure prediction empowers integrative structural analysis of human nuclear pores (2022) · cites this paper as [59]
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AI-based structure prediction empowers integrative structural analysis of human nuclear pores
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- Authors
- Otsuka, S., Steyer, A. M., Schorb, M., Hériché, J.-K., Hossain, M. J., Sethi, S., Kueblbeck, M., Schwab, Y., Beck, M., & Ellenberg, J.
- Affiliations
- European Molecular Biology Laboratory (DE)
- Corresponding author
- Ellenberg, Jan
- ORCID
- Venue
- Nature Structural & Molecular Biology
- Published
- 2017-11-27
- Volume
- 25
- Issue
- 1
- Pages
- 21-28
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W2949721790
- Cited by
- 90
- References
- 38
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 9 · 2023: 12 · 2022: 10 · 2021: 10 · 2020: 16 · 2019: 9 · 2018: 7 · 2017: 2
- FWCI
- 3.80
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- Nature Structural & Molecular Biology (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- Baden-Württemberg Stiftung
- European Molecular Biology Laboratory
- Deutsche Forschungsgemeinschaft (SPP1175)
- Japan Society for the Promotion of Science
- FP7 Ideas: European Research Council (309271)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- nuclear pore complex assembly, nuclear envelope reassembly, mitosis, endoplasmic reticulum, membrane sealing
- MeSH terms
- Gene Editing, Animals, Cell Membrane, Cell Nucleus, Chromosomes, Cytoplasm, Endoplasmic Reticulum, HeLa Cells, Humans, Interphase, Kinetics, Microscopy, Electron, Scanning, Mitosis, Nuclear Envelope, Xenopus, Nuclear Pore, Nuclear Pore Complex Proteins, Electron Microscope Tomography
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