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Cited by 25RNA and protein synthesis mechanismsNuclear Structure and FunctionGenomics and Chromatin Dynamics

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A co-transcriptional ribosome assembly checkpoint controls nascent large ribosomal subunit maturation

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Sanghai, Zahra A., Piwowarczyk, Rafal, Broeck, Arnaud Vanden, Klinge, Sebastian·2023·Nature Structural & Molecular Biology
DOI 10.1038/s41594-023-00947-3PMC PMC10191850

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Abstract

During transcription of eukaryotic ribosomal DNA in the nucleolus, assembly checkpoints exist that guarantee the formation of stable precursors of small and large ribosomal subunits. While the formation of an early large subunit assembly checkpoint precedes the separation of small and large subunit maturation, its mechanism of action and function remain unknown. Here, we report the cryo-electron microscopy structure of the yeast co-transcriptional large ribosomal subunit assembly intermediate that serves as a checkpoint. The structure provides the mechanistic basis for how quality-control pathways are established through co-transcriptional ribosome assembly factors, that structurally interrogate, remodel and, together with ribosomal proteins, cooperatively stabilize correctly folded pre-ribosomal RNA. Our findings thus provide a molecular explanation for quality control during eukaryotic ribosome assembly in the nucleolus.

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Metadata

Authors
Sanghai, Z. A., Piwowarczyk, R., Broeck, A. V., & Klinge, S.
Affiliations
Rockefeller University (US)
Corresponding author
Klinge, Sebastian
ORCID
Venue
Nature Structural & Molecular Biology
Publisher
Springer Science and Business Media LLC
Published
2023-04-10
Volume
30
Issue
5
Pages
594-599
ISSN
  • 1545-9985 (ISSN-L)
  • 1545-9993
DOI
10.1038/s41594-023-00947-3
PMC
PMC10191850
OpenAlex
W4363673446
Cited by
25
References
1
Citations by year
2026: 6 · 2025: 5 · 2024: 11 · 2023: 3
FWCI
2.23
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Structural & Molecular Biology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • ORBi (University of Liège) (submittedVersion, cc-by-nc-nd)
Funders
  • Leona M. and Harry B. Helmsley Charitable Trust
  • G. Harold and Leila Y. Mathers Foundation
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM143181)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
ribosomal DNA transcription, nucleolus, quality control, yeast, ribosomal proteins
MeSH terms
Ribosomal Proteins, RNA, Ribosomal, Saccharomyces cerevisiae, Cryoelectron Microscopy, Saccharomyces cerevisiae Proteins, Ribosome Subunits, Large, Ribosome Subunits, Small, Eukaryotic, Ribosome Subunits, Large, Eukaryotic

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