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Cited by 23Poxvirus research and outbreaksAdvanced Electron Microscopy Techniques and ApplicationsEnzyme Structure and Function

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Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores

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Datler, Julia, Hansen, Jesse M., Thader, Andreas, Schlögl, Alois, Bauer, Lukas W., Hodirnau, Victor-Valentin, Schur, Florian K. M.·2024·Nature Structural & Molecular Biology
DOI 10.1038/s41594-023-01201-6PMC PMC11257981

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Abstract

Poxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural proteins that form the viral core has remained sparse. While major core proteins have been annotated via indirect experimental evidence, their structures have remained elusive and they could not be assigned to individual core features. Hence, which proteins constitute which layers of the core, such as the palisade layer and the inner core wall, has remained enigmatic. Here we show, using a multi-modal cryo-electron microscopy (cryo-EM) approach in combination with AlphaFold molecular modeling, that trimers formed by the cleavage product of VACV protein A10 are the key component of the palisade layer. This allows us to place previously obtained descriptions of protein interactions within the core wall into perspective and to provide a detailed model of poxvirus core architecture. Importantly, we show that interactions within A10 trimers are likely generalizable over members of orthopox- and parapoxviruses.

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Metadata

Authors
Datler, J., Hansen, J. M., Thader, A., Schlögl, A., Bauer, L. W., Hodirnau, V.-V., & Schur, F. K. M.
Affiliations
Institute of Science and Technology Austria (AT)
Corresponding author
Schur, Florian K. M.
ORCID
Venue
Nature Structural & Molecular Biology
Publisher
Springer Science and Business Media LLC
Published
2024-02-05
Volume
31
Issue
7
Pages
1114-1123
ISSN
  • 1545-9985 (ISSN-L)
  • 1545-9993
DOI
10.1038/s41594-023-01201-6
PMC
PMC11257981
OpenAlex
W4391540113
Cited by
23
References
5
Citations by year
2026: 11 · 2025: 3 · 2024: 9
FWCI
5.28
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Structural & Molecular Biology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Silicon Valley Community Foundation (10.13039/100014989)
  • Institute of Science and Technology Austria
  • Chan Zuckerberg Initiative
  • Österreichischen Akademie der Wissenschaften
  • Austrian Science Fund (P31445)
  • Medizinische Universität Wien
Topics
  • Life Sciences › Immunology and Microbiology › Virology › Poxvirus research and outbreaks
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
Keywords
poxvirus, vaccinia virus, viral core structure, palisade layer, core wall, orthopoxvirus, viral structural proteins
MeSH terms
Humans, Models, Molecular, Poxviridae, Vaccinia virus, Viral Core Proteins, Cryoelectron Microscopy, Protein Multimerization

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