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PEG-4MAL hydrogels for human organoid generation, culture, and in vivo delivery
Open accessAbstract
In vitro differentiation of human pluripotent stem cell (hPSC)-derived organoids (HOs) facilitates the production of multicellular three-dimensional structures analogous to native human tissues. Most current methods for the generation of HOs rely on Matrigel, a poorly defined basement membrane derivative secreted by Engelbreth-Holm-Swarm mouse sarcoma cells, limiting the potential use of HOs for regenerative medicine applications. Here, we describe a protocol for the synthesis of a fully defined, synthetic hydrogel that supports the generation and culture of HOs. Modular, cell-encapsulating hydrogels are formed from a four-armed poly(ethylene glycol) macromer that has maleimide groups at each terminus (PEG-4MAL) and is conjugated to cysteine-containing adhesive peptides and cross-linked via protease-degradable peptides. The protocol also includes guidelines for the localized in vivo delivery of PEG-4MAL hydrogel-encapsulated HOs to injured mouse colon. The PEG-4MAL hydrogel supports the engraftment of the HOs and accelerates colonic wound repair. This culture and delivery strategy can thus be used to develop HO-based therapies to treat injury and disease. Hydrogel and tissue preparation and subsequent encapsulation can be performed within 2.5-3.5 h. Once HOs have been cultured in synthetic hydrogels for at least 14 d, they can be prepared and delivered to the mouse colon in under 5 h.
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“Intriguingly, when PEG was crosslinked with dithiothreitol to inhibit matrix degradation, organoid viability at seven days was poor as measured by live-dead staining.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [164]
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“Organoid viability was reduced at high PEG density and matrix stiffness;”
From Towards organoid culture without Matrigel (2021) · cites this paper as [164]
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“RGD- or AG73 (CGGRKRLQVQLSIRT)-functionalized PEG matrices promoted greater organoid viability than laminin-derived IKVAV- or type I collagen-derived GFOGER peptides163,164.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [164]
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“Cruz-Acuna and colleagues also found that PEG-based materials may need to be soft and degradable in order to support differentiated intestinal organoids.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [164]
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Citations
Cites
Organoid Models and Applications in Biomedical Research
Xinaris, C., Brizi, V., & Remuzzi, G. (2015) · The Nephron journals/Nephron journals · doi:10.1159/000433566 · Cited by 3,519
Modeling Development and Disease with Organoids
Clevers, H. (2016) · Cell · doi:10.1016/j.cell.2016.05.082 · Cited by 3,196
Extracellular Matrix Degradation and Remodeling in Development and Disease
Lu, P., Takai, K., Weaver, V. M., & Werb, Z. (2011) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a005058 · Cited by 2,265
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro
Spence, J. R., Mayhew, C. N., Rankin, S. A., Kuhar, M. F., Vallance, J. E., Tolle, K. A., Hoskins, E. E., Kalinichenko, V. V., Wells, S. I., Zorn, A. M., et al. (2010) · Nature · doi:10.1038/nature09691 · Cited by 1,911
Self-citationGeneration of cerebral organoids from human pluripotent stem cells
Lancaster, M. A., & Knoblich, J. A. (2014) · Nature Protocols · doi:10.1038/nprot.2014.158 · Cited by 1,823
Matrigel: A complex protein mixture required for optimal growth of cell culture
Hughes, C. S., Postovit, L. M., & Lajoie, G. A. (2010) · PROTEOMICS · doi:10.1002/pmic.200900758 · Cited by 1,534
Organoids as an in vitro model of human development and disease
Fatehullah, A., Tan, S. H., & Barker, N. (2016) · Nature Cell Biology · doi:10.1038/ncb3312 · Cited by 1,508
Bioactive modification of poly (ethylene glycol) hydrogels for tissue engineering
Zhu, J. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.02.044 · Cited by 1,457
Self-formation of optic cups and storable stratified neural retina from human ESCs
Nakano, T., Ando, S., Takata, N., Kawada, M., Muguruma, K., Sekiguchi, K., Saito, K., Yonemura, S., Eiraku, M., & Sasai, Y. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.05.009 · Cited by 1,447
Designer matrices for intestinal stem cell and organoid culture
Gjorevski, N., Sachs, N., Manfrin, A., Giger, S., Bragina, M. E., Ordóñez-Morán, P., Clevers, H., & Lutolf, M. P. (2016) · Nature · doi:10.1038/nature20168 · Cited by 1,426
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Daoud, A., & Múnera, J. O. (2019) · Frontiers in Medicine · doi:10.3389/fmed.2019.00297 · Cited by 11
Macdougall, L. J., & Anseth, K. S. (2020) · Macromolecules · doi:10.1021/acs.macromol.0c00030 · Cited by 11
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Cai, Y., Nan, F., Tang, G.-T., Ma, Y., Ren, Y., Xiong, X., Zhou, R., Li, F., Cheng, N.-S., & Jiang, X. (2023) · Journal of Materials Chemistry B · doi:10.1039/d3tb01250f · Cited by 10
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Feng, P., He, C., Li, G., Li, J., Luo, Y. H., Chen, Y., Tang, Y., Ma, J., & Ke, C. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.139711 · Cited by 9
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Wodzanowski, K. A., Cassel, S. E., Grimes, C. L., & Kloxin, A. M. (2020) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2020.127116 · Cited by 9
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Kim, N. H., Chae, S., Yi, S. A., Sa, D. H., Oh, S., Kang, E. S., Kim, S., Choi, K. H., Lee, J., Choi, J., et al. (2023) · Nano Letters · doi:10.1021/acs.nanolett.3c00966 · Cited by 7
Liu, D., Wagner, E. K., Lakhotia, S., He, H., Laflin, A. D., Liu, P., Khetan, S., Tang, C., Chen, S., & Jiang, S. (2025) · Biomaterials · doi:10.1016/j.biomaterials.2025.123825 · Cited by 5
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Thai, V. L., Candelas, D. O., & Leach, J. K. (2023) · Annals of Biomedical Engineering · doi:10.1007/s10439-023-03162-9 · Cited by 5
Park, S.-H., Kwon, O., Lee, H., Cho, Y., Yeun, J., Yoon, S., Sun, S. Y., Huh, Y., Yu, W. D., Park, S., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-54653-9 · Cited by 7
Slaby, E. M., Hansen, N. F., Sharma, R., Pirrotte, P., & Weaver, J. D. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.05.13.594007 · Cited by 5
Giri, S., & Rajesh, P. (2026) · Biomedicines · doi:10.3390/biomedicines14020485 · Cited by 5
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Hydrogel design for intestinal organoids: principles governing translational regenerative medicine
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Current knowledge on the multiform reconstitution of intestinal stem cell niche
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Gao, Y., Zhang, Y., Wang, Y., Liu, B., Liu, F., & Ba, X. (2023) · Cellulose · doi:10.1007/s10570-023-05509-w · Cited by 2
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Ebrahim, N. A., Mwizerwa, O., Ekwueme, E. C., Muss, T. E., Ersland, E. E., Oba, T., Oku, K., Nishino, M., Hikimoto, D., Miyoshi, H., et al. (2023) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.37517 · Cited by 1
Bulondo, F., & Babensee, J. E. (2024) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.37714 · Cited by 1
Ray, P. G., Rajasekaran, R., Roy, T., Dutta, A., Saha, B., Bora, H., Das, S. K., & Dhara, S. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-821085-7.00003-8 · Cited by 1
Siddiqui, S. S., Aboshamat, K., Loganathan, S., & Siddiqui, Z. K. (2021) · Stem cell biology and regenerative medicine · doi:10.1007/978-3-030-78101-9_4 · Cited by 1
Lopreside, A., Calabretta, M. M., Montali, L., Roda, A., & Michelini, E. (2021) · Springer eBooks · doi:10.1007/978-3-030-23217-7_146 · Cited by 1
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Lim, H. Y. G., Kostic, L., & Barker, N. (2022) · Advances in stem cells and their niches · doi:10.1016/bs.asn.2021.10.001 · Cited by 1
Lopreside, A., Calabretta, M. M., Montali, L., Roda, A., & Michelini, E. (2019) · Springer eBooks · doi:10.1007/978-3-319-47405-2_146-1
Cruz-Acuña, R., Kariuki, S. W., Sugiura, K., Loebel, C., Karakasheva, T. A., Gabre, J., Burdick, J., & Rustgi, A. (2022) · bioRxiv · doi:10.1101/2022.11.13.516357
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Zhou, L., Liu, D., Wei, J., Zhu, X., & Jiang, L. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4581586
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Metadata
- Authors
- Cruz-Acuña, R., Quirós, M., Huang, S., Siuda, D., Spence, J. R., Nusrat, A., & García, A. J.
- Affiliations
- University of Michigan (US)
- Corresponding author
- Spence, Jason R.
- Nusrat, Asma
- Venue
- Nature Protocols
- Published
- 2018-09
- Volume
- 13
- Issue
- 9
- Pages
- 2102-2119
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- PMC
- PMC7240347
- OpenAlex
- W2892378739
- Cited by
- 168
- References
- 30
- Citations by year
- 2026: 16 · 2025: 26 · 2024: 16 · 2023: 24 · 2022: 26 · 2021: 30 · 2020: 16 · 2019: 14
- FWCI
- 5.67
- Type
- journal article
- Language
- en
- Update notices
- correction (2018-10-24)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Protocols (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (UL1TR000454)
- National Heart, Lung, and Blood Institute (R01HL119215)
- National Institute of Allergy and Infectious Diseases (U19AI116482)
- National Institute of Diabetes and Digestive and Kidney Diseases (U24DK085532, P30DK034933, R01DK055679, R56DK089763, R29DK055679, R01DK059888, U01DK085532, U01DK103141, R01DK089763)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR062368, R01AR062920)
- National Center for Advancing Translational Sciences
- Topics
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- human organoids, pluripotent stem cell differentiation, synthetic hydrogels, hydrogel encapsulation, in vivo delivery, tissue regeneration
- MeSH terms
- Animals, Colon, Humans, Organoids, Organ Transplantation, Hydrogels, Models, Animal, Pluripotent Stem Cells, Tissue Culture Techniques, Mice
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