The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results
Open accessAbstract
The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between ‘desirable’ and ‘essential’ information: ‘essential’ information refers to the precise details that are necessary to assess the quality of the experimental work, whereas ‘desirable’ information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.
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The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G. L., et al. (2009) · Clinical Chemistry · doi:10.1373/clinchem.2008.112797 · Cited by 16,303
Minimum information about a microarray experiment (MIAME)-toward standards for microarray data
Brazma, A., Hingamp, P., Quackenbush, J., Sherlock, G., Spellman, P., Stoeckert, C., Aach, J., Ansorge, W., Ball, C. A., Causton, H. C., et al. (2001) · Nature Genetics · doi:10.1038/ng1201-365 · Cited by 4,109
Olive, P. L., Banáth, J. P., Durand, R. E., & Banath, J. P. (1990) · Radiation Research · doi:10.2307/3577587 · Cited by 1,094
Olive, P. L., Banáth, J. P., & Durand, R. E. (2012) · Radiation Research · doi:10.1667/rrav04.1 · Cited by 1,029
The comet assay: topical issues
Collins, A. R., Oscoz, A. A., Brunborg, G., Gaivao, I., Giovannelli, L., Kruszewski, M., Smith, C. C., & Stetina, R. (2008) · Mutagenesis · doi:10.1093/mutage/gem051 · Cited by 991
Self-citationESCODD (European Standards Committee on Oxidative DNA Damage), Gedik, C. M., & Collins, A. (2005) · The FASEB Journal · doi:10.1096/fj.04-1767fje · Cited by 449
Self-citationFactors influencing DNA migration from individual cells subjected to gel electrophoresis
Olive, P. L., Wlodek, D., Durand, R. E., & Banáth, J. P. (1992) · Experimental Cell Research · doi:10.1016/0014-4827(92)90378-l · Cited by 364
Statistical issues in the use of the comet assay
Lovell, D. P., & Omori, T. (2008) · Mutagenesis · doi:10.1093/mutage/gen015 · Cited by 297
Measurement of DNA oxidation in human cells by chromatographic and enzymic methods
(2003) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(03)00041-8 · Cited by 285
Cited by
Do cytotoxicity and cell death cause false positive results in the in vitro comet assay?
Azqueta, A., Stopper, H., Zegura, B., Dusinska, M., & Møller, P. (2022) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2022.503520 · Cited by 80
Self-citationGenome-wide mapping of genomic DNA damage: methods and implications
Amente, S., Scala, G., Majello, B., Azmoun, S., Tempest, H. G., Premi, S., & Cooke, M. S. (2021) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-021-03923-6 · Cited by 33
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Metadata
- Authors
- Møller, P., Azqueta, A., Boutet-Robinet, E., Koppen, G., Bonassi, S., Milić, M., Gajski, G., Costa, S., Teixeira, J. P., Costa Pereira, C., Dusinska, M., Godschalk, R., Brunborg, G., Gutzkow, K. B., Giovannelli, L., Cooke, M. S., Richling, E., Laffon, B., Valdiglesias, V., Basaran, N., Del Bo’, C., Zegura, B., Novak, M., Stopper, H., Vodicka, P., Vodenkova, S., de Andrade, V. M., Sramkova, M., Gabelova, A., Collins, A., & Langie, S. A. S.
- Venue
- Nature Protocols
- Published
- 2020-10-26
- Volume
- 15
- Issue
- 12
- Pages
- 3817-3826
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W3093583810
- Cited by
- 394
- References
- 36
- FWCI
- 13.88
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Møller, P., Azqueta, A., Boutet-Robinet, E., Koppen, G., Bonassi, S., Milić, M., Gajski, G., Costa, S., Teixeira, J. P., Costa Pereira, C., Dusinska, M., Godschalk, R., Brunborg, G., Gutzkow, K. B., Giovannelli, L., Cooke, M. S., Richling, E., Laffon, B., Valdiglesias, V., . . . Langie, S. A. S. (2020). Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. Nature Protocols. https://doi.org/10.1038/s41596-020-0398-1
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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