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Cited by 28

Spatial transcriptome profiling of normal human liver

Yu, Shizhe, Wang, Haoren, Yang, Lingpeng, Yan, Yingxue, Cai, Qiang, Ma, Duo, Jiang, Long, Gao, Zehai +2 more·2022·Scientific Data
DOI 10.1038/s41597-022-01676-wPMC PMC9581974
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Abstract

The comprehensive study of the spatial-cellular anatomy of the human liver is critical to addressing the cellular origins of liver disease. Here we conducted spatial transcriptomics on normal human liver tissue sections, providing detailed information of liver zonation at the transcriptional level. We present 6581 high-quality spots from normal livers of two human donors. In this dataset, cells were mainly hepatocytes, and we classified them into four sub-groups. Collectively, these data provide a reliable reference for studies on spatial heterogeneity of liver lobules.

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  • Expression of perivenous markers was significantly elevated close to the outlet where oxygenation is lower.

    “In contrast, expression of perivenous markers was significantly elevated close to the outlet where oxygenation is lower.”

    From 3D microperfusion of mesoscale human microphysiological liver models improves functionality and recapitulates hepatic zonation (2023) · cites this paper as [35]

    Technical Validation identifies GLUL, CYP3A4, and CYP1A1 as peri-central genes near the central veins, supporting the location of perivenous marker expression. The passage does not report that their expression was significantly elevated, and Background & Summary describes differing environments along the lobule without stating that oxygenation is lower near the central veins. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Close to the inlet, where oxygen concentrations are highest, increased levels of the periportal marker proteins ARG1, BAAT, PYGL, FBP1 and ASL were observed.

    “Close to the inlet, where oxygen concentrations are highest, we observed increased levels of the periportal marker proteins ARG1, BAAT, PYGL, FBP1 and ASL ( Fig. 6 a).”

    From 3D microperfusion of mesoscale human microphysiological liver models improves functionality and recapitulates hepatic zonation (2023) · cites this paper as [35]

    The Technical Validation section identifies zone 1 near the portal veins and reports periportal enrichment of HAL, HAMP and CRP. It does not report increased ARG1, BAAT, PYGL, FBP1 or ASL protein levels, or measure oxygen concentrations; the citing sentence describes those observations as its own. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

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Research works printed but not linked: 20

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Cited by

Sorted by citations

Authors
Yu, S., Wang, H., Yang, L., Yan, Y., Cai, Q., Ma, D., Jiang, L., Gao, Z., Yu, Z., & Xia, Z.
Venue
Scientific Data
Publisher
Springer Science and Business Media LLC
Published
2022-10-19
Volume
9
Issue
1
ISSN
2052-4463
DOI
10.1038/s41597-022-01676-w
PMC
PMC9581974
Cited by
28
References
5
Language
en
Licence

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