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Cited by 48MicroRNA in disease regulationAngiogenesis and VEGF in CancerTelomeres, Telomerase, and Senescence

MicroRNA profiling reveals distinct profiles for tissue-derived and cultured endothelial cells

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Kuosmanen, Suvi M., Kansanen, Emilia, Sihvola, Virve, Levonen, Anna-Liisa·2017·Scientific Reports
DOI 10.1038/s41598-017-11487-4PMC PMC5591252
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Abstract

Endothelial plasticity enables the cells to switch their phenotype according to the surrounding vascular microenvironment. MicroRNAs (miRNAs) are small noncoding RNAs that control endothelial plasticity. The objective of this study was to investigate the differences in miRNA profiles of tissue-derived cells and cultured endothelial cells. To this end, miRNA expression was profiled from freshly isolated tissue-derived human vascular endothelial cells and endothelial cells cultured until cellular senescence using miRNA sequencing. In addition, the data was searched for putative novel endothelial miRNAs and miRNA isoforms. The data analysis revealed a striking change in endothelial miRNA profile as the cells adapted from tissue to cell culture environment and the overall miRNA expression decreased significantly in cultured compared to tissue-derived endothelial cells. In addition to changes in mechanosensitive miRNA expression, alterations in senescence-associated and endothelial-to-mesenchymal-transition-associated miRNAs were observed in aging cells. Collectively, the data illustrates the adaptability of endothelial cell miRNA expression that mirrors prevailing cellular environment.

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  • Ex vivo conditions are known to sometimes dramatically alter miRNA expression patterns, as seen for passaged endothelial cells.

    “Ex vivo conditions are known to sometimes dramatically alter miRNA expression patterns, as seen for passaged endothelial cells [24].”

    From A curated human cellular microRNAome based on 196 primary cell types (2022) · cites this paper as [24]

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Cited by

Sorted by citations

Authors
Kuosmanen, S. M., Kansanen, E., Sihvola, V., & Levonen, A.-L.
Affiliations
University of Eastern Finland (FI)
Corresponding author
Levonen, Anna-Liisa
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2017-09-08
Volume
7
Issue
1
Pages
10943-10943
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-017-11487-4
PMC
PMC5591252
OpenAlex
W2753544039
Cited by
48
References
6
Citations by year
2026: 1 · 2025: 3 · 2024: 2 · 2023: 7 · 2022: 10 · 2021: 6 · 2020: 6 · 2019: 4 · 2018: 8 · 2017: 1
FWCI
1.76
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • UEF eRepo (University of Eastern Finland) (submittedVersion, cc-by)
Funders
Academy of Finland
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
Keywords
miRNA expression profiling, endothelial plasticity, cultured endothelial cells, cellular senescence, endothelial-to-mesenchymal transition, novel miRNAs, miRNA isoforms, vascular microenvironment
MeSH terms
Humans, Cellular Senescence, Gene Expression Profiling, MicroRNAs, Mechanotransduction, Cellular, Transcriptome, Human Umbilical Vein Endothelial Cells

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