The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
CRISPR/Cas9-induced Targeted Mutagenesis and Gene Replacement to Generate Long-shelf Life Tomato Lines
Open accessAbstract
Quickly and precisely gain genetically enhanced breeding elites with value-adding performance traits is desired by the crop breeders all the time. The present of gene editing technologies, especially the CRISPR/Cas9 system with the capacities of efficiency, versatility and multiplexing provides a reasonable expectation towards breeding goals. For exploiting possible application to accelerate the speed of process at breeding by CRISPR/Cas9 technology, in this study, the Agrobacterium tumefaciens-mediated CRISPR/Cas9 system transformation method was used for obtaining tomato ALC gene mutagenesis and replacement, in absence and presence of the homologous repair template. The average mutation frequency (72.73%) and low replacement efficiency (7.69%) were achieved in T 0 transgenic plants respectively. None of homozygous mutation was detected in T 0 transgenic plants, but one plant carry the heterozygous genes (Cas9/*-ALC/alc) was stably transmitted to T 1 generations for segregation and genotyping. Finally, the desired alc homozygous mutants without T-DNA insertion (*/*-alc/alc) in T 1 generations were acquired and further confirmed by genotype and phenotype characterization, with highlight of excellent storage performance, thus the recessive homozygous breeding elites with the character of long-shelf life were generated. Our results support that CRISPR/Cas9-induced gene replacement via HDR provides a valuable method for breeding elite innovation in tomato.
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Cites
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Self-citationBreeding Technologies to Increase Crop Production in a Changing World
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Targeted genome modification of crop plants using a CRISPR-Cas system
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Cited by
CRISPR for Crop Improvement: An Update Review
Jaganathan, D., Ramasamy, K., Sellamuthu, G., Jayabalan, S., & Venkataraman, G. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00985 · Cited by 619
Modrzejewski, D., Hartung, F., Lehnert, H., Sprink, T., Kohl, C., Keilwagen, J., & Wilhelm, R. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.574959 · Cited by 151
Base Editing in Plants: Applications, Challenges, and Future Prospects
Azameti, M. K., & Dauda, W. P. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.664997 · Cited by 88
Concerns regarding ‘off-target’activity of genome editing endonucleases
Kadam, U. S., Shelake, R. M., Chavhan, R. L., & Suprasanna, P. (2018) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2018.03.027 · Cited by 47
Revisiting CRISPR/Cas-mediated crop improvement: special focus on nutrition
Kaul, T., Sony, S. K., Verma, R., Motelb, K. F. A., Prakash, A. T., Eswaran, M., Bharti, J., Nehra, M., & Kaul, R. (2020) · Journal of Biosciences · doi:10.1007/s12038-020-00094-7 · Cited by 33
Metadata
- Authors
- Yu, Q.-H., Wang, B., Li, N., Tang, Y., Yang, S., Yang, T., Xu, J., Guo, C., Yan, P., Wang, Q., & Asmutola, P.
- Venue
- Scientific Reports
- Published
- 2017-09-19
- Volume
- 7
- Issue
- 1
- Pages
- 11874-11874
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2756408218
- Cited by
- 258
- References
- 38
- FWCI
- 6.21
- Type
- journal article
- Language
- en
- Open access
- gold
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- Yu, Q.-H., Wang, B., Li, N., Tang, Y., Yang, S., Yang, T., Xu, J., Guo, C., Yan, P., Wang, Q., & Asmutola, P. (2017). CRISPR/Cas9-induced Targeted Mutagenesis and Gene Replacement to Generate Long-shelf Life Tomato Lines. Scientific Reports. https://doi.org/10.1038/s41598-017-12262-1
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