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Targeted genome modification of crop plants using a CRISPR-Cas system
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CRISPR-mediated manipulation has been applied to all sorts of cells and some multicellular organisms, such as human cell cultures, mice, plants, and yeasts.
“Soon, all sorts of cells and some multicellular organisms would be the object of CRISPR-mediated manipulation, such as human cell cultures, mice, plants, yeasts, and the list goes on [62,63,64,65].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [64]
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“Although CRISPR-Cas9 has revolutionized our ability to generate site-specific double-strand breaks, precise editing of the genome remains challenging in most eukaryotes, including plants (Shan et al., 2013).”
BackgroundThe CRISPR/Cas9 system and its applications in crop genome editing
Bao, A., Burritt, D. J., Chen, H., Zhou, X., Cao, D., & Tran, L.-S. P. (2019) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2018.1554621 · Cited by 185
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“The CRISPR/Cas9 system has emerged as the most powerful tool for GE in several organisms, including model and non-model plant species [91].”
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“In several plants, continuous innovation for efficient genome editing has expanded the application of CRISPR-Cas9 and is rapidly becoming a promising tool for gene modifications (Shan et al., 2013; Zhang et al., 2019).”
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Metadata
- Authors
- Shan, Q., Wang, Y., Li, J., Zhang, Y., Chen, K., Liang, Z., Zhang, K., Liu, J., Xi, J. J., Qiu, J.-L., & Gao, C.
- Venue
- Nature Biotechnology
- Published
- 2013-08
- Volume
- 31
- Issue
- 8
- Pages
- 686-688
- ISSN
- 1087-0156
- 1546-1696
- Cited by
- 1,810
- References
- 6
- Language
- en
- Licence
- www.springer.com/tdm (tdm)
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