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Cited by 276Neuroinflammation and Neurodegeneration MechanismsTraumatic Brain Injury and Neurovascular DisturbancesTraumatic Brain Injury Research

Quantitative microglia analyses reveal diverse morphologic responses in the rat cortex after diffuse brain injury

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Morrison, Helena, Young, Kimberly, Qureshi, Mahir, Rowe, Rachel K., Lifshitz, Jonathan·2017·Scientific Reports
DOI 10.1038/s41598-017-13581-zPMC PMC5643511
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Abstract

Determining regions of altered brain physiology after diffuse brain injury is challenging. Microglia, brain immune cells with ramified and dynamically moving processes, constantly surveil the parenchyma for dysfunction which, when present, results in a changed morphology. Our purpose was to define the spatiotemporal changes in microglia morphology over 28 days following rat midline fluid percussion injury (mFPI) as a first step in exploiting microglia morphology to reflect altered brain physiology. Microglia morphology was quantified from histological sections using Image J skeleton and fractal analysis procedures at three time points and in three regions post-mFPI: impact site, primary somatosensory cortex barrel field (S1BF), and a remote region. Microglia ramification (process length/cell and endpoints/cell) decreased in the impact and S1BF but not the remote region (p < 0.05). Microglia complexity was decreased in the S1BF (p = 0.003) and increased in the remote region (p < 0.02). Rod-shaped microglia were present in the S1BF and had a 1.8:1.0 length:width ratio. An in-depth quantitative morphologic analysis revealed diverse and widespread changes to microglia morphology in the cortex post-mFPI. Due to their close link to neuronal function, changes in microglia morphology, summarized in this study, likely reflect altered physiology with diverse and widespread impact on neuronal and circuit function.

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Cited by

Sorted by citations

Authors
Morrison, H., Young, K., Qureshi, M., Rowe, R. K., & Lifshitz, J.
Affiliations
  • University of Arizona (US)
  • Arizona State University (US)
  • Barrow Neurological Institute (US)
Corresponding author
Morrison, Helena
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2017-10-16
Volume
7
Issue
1
Pages
13211-13211
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-017-13581-z
PMC
PMC5643511
OpenAlex
W2761242880
Cited by
276
References
8
Citations by year
2026: 19 · 2025: 42 · 2024: 39 · 2023: 41 · 2022: 45 · 2021: 43 · 2020: 22 · 2019: 18 · 2018: 7
FWCI
6.40
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • UA Campus Repository (The University of Arizona) (acceptedVersion, cc-by)
Funders
  • Science Foundation Arizona
  • University of Arizona
  • Diane and Bruce Halle Foundation
  • National Institutes of Health
  • National Institute of Neurological Disorders and Stroke (R03NS090013, R21NS096515)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
  • Health Sciences › Medicine › Epidemiology › Traumatic Brain Injury Research
Keywords
microglial morphology, diffuse brain injury, fluid percussion injury, primary somatosensory cortex, barrel cortex, microglial ramification, fractal analysis, neuroinflammation
MeSH terms
Brain Injuries, Diffuse, Animals, Male, Percussion, Somatosensory Cortex, Rats, Sprague-Dawley, Microglia, Fractals, Rats

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