The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Nanopore DNA Sequencing and Genome Assembly on the International Space Station
Open accessAbstract
We evaluated the performance of the MinION DNA sequencer in-flight on the International Space Station (ISS), and benchmarked its performance off-Earth against the MinION, Illumina MiSeq, and PacBio RS II sequencing platforms in terrestrial laboratories. Samples contained equimolar mixtures of genomic DNA from lambda bacteriophage, Escherichia coli (strain K12, MG1655) and Mus musculus (female BALB/c mouse). Nine sequencing runs were performed aboard the ISS over a 6-month period, yielding a total of 276,882 reads with no apparent decrease in performance over time. From sequence data collected aboard the ISS, we constructed directed assemblies of the ~4.6 Mb E. coli genome, ~48.5 kb lambda genome, and a representative M. musculus sequence (the ~16.3 kb mitochondrial genome), at 100%, 100%, and 96.7% consensus pairwise identity, respectively; de novo assembly of the E. coli genome from raw reads yielded a single contig comprising 99.9% of the genome at 98.6% consensus pairwise identity. Simulated real-time analyses of in-flight sequence data using an automated bioinformatic pipeline and laptop-based genomic assembly demonstrated the feasibility of sequencing analysis and microbial identification aboard the ISS. These findings illustrate the potential for sequencing applications including disease diagnosis, environmental monitoring, and elucidating the molecular basis for how organisms respond to spaceflight.
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Cites
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Cited by
Nanopore sequencing technology, bioinformatics and applications
Wang, Y., Zhao, Y., Bollas, A., Wang, Y., & Au, K. F. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-01108-x · Cited by 1,753
Chiu, C. Y., & Miller, S. A. (2019) · Nature Reviews Genetics · doi:10.1038/s41576-019-0113-7 · Cited by 1,620
Self-citationThe Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community
Jain, M., Olsen, H. E., Paten, B., & Akeson, M. (2016) · Genome Biology · doi:10.1186/s13059-016-1103-0 · Cited by 1,588
Towards a genomics-informed, real-time, global pathogen surveillance system
Gardy, J. L., & Loman, N. J. (2018) · Nature Reviews Genetics · doi:10.1038/nrg.2017.88 · Cited by 771
Fundamental Biological Features of Spaceflight: Advancing the Field to Enable Deep-Space Exploration
Afshinnekoo, E., Scott, R. T., MacKay, M. J., Pariset, E., Cekanaviciute, E., Barker, R., Gilroy, S., Hassane, D., Smith, S. M., Zwart, S. R., et al. (2020) · Cell · doi:10.1016/j.cell.2020.10.050 · Cited by 478
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Metadata
- Authors
- Castro-Wallace, S. L., Chiu, C. Y., John, K. K., Stahl, S. E., Rubins, K. H., McIntyre, A. B. R., Dworkin, J. P., Lupisella, M. L., Smith, D. J., Botkin, D. J., Stephenson, T. A., Juul, S., Turner, D. J., Izquierdo, F., Federman, S., Stryke, D., Somasekar, S., Alexander, N., Yu, G., Mason, C. E., & Burton, A. S.
- Venue
- Scientific Reports
- Published
- 2017-12-21
- Volume
- 7
- Issue
- 1
- Pages
- 18022-18022
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2949303487
- Cited by
- 375
- References
- 40
- FWCI
- 15.63
- Type
- journal article
- Language
- en
- Open access
- gold
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- Castro-Wallace, S. L., Chiu, C. Y., John, K. K., Stahl, S. E., Rubins, K. H., McIntyre, A. B. R., Dworkin, J. P., Lupisella, M. L., Smith, D. J., Botkin, D. J., Stephenson, T. A., Juul, S., Turner, D. J., Izquierdo, F., Federman, S., Stryke, D., Somasekar, S., Alexander, N., Yu, G., . . . Burton, A. S. (2017). Nanopore DNA Sequencing and Genome Assembly on the International Space Station. Scientific Reports. https://doi.org/10.1038/s41598-017-18364-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
