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Cited by 109Algal biology and biofuel productionLipid metabolism and biosynthesisMicrobial Metabolic Engineering and Bioproduction

Transcriptome analysis for phosphorus starvation-induced lipid accumulation in Scenedesmus sp

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Yang, Fangfang, Xiang, Wenzhou, Li, Tao, Long, Lijuan·2018·Scientific Reports
DOI 10.1038/s41598-018-34650-xPMC PMC6219579
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Abstract

Phosphorus stress was applied to enhance the lipid production in Scenedesmus sp. The highest lipid production (350 mg L−1) and lipid content (approximately 41.0% of dry weight) were obtained by addition of 2 mg L−1 NaH 2 PO 4·2H 2 O every 2 days, which were higher than those in replete phosphorus. Correspondingly, carbohydrate content decreased significantly. We speculated that phosphorus limitation could block starch biosynthesis, and photosynthate flow tended to fatty acid biosynthesis to cope with stress. To investigate the mechanism that phosphorus stress triggers the carbon fixation to lipid biosynthesis, the transcriptome analysis was carried out by the Illumina RNA-seq platform. A total of 2897 genes were identified as differentially expressed genes. The observed overexpression of lipid production under phosphorus stress was bolstered by up-regulation of genes encoding for DGAT and pyruvate kinase, activation of carbohydrate metabolism pathway and fatty acid biosynthesis, and repression of carbohydrate synthesis-presumably to shunt the carbon flux toward TAG biosynthesis. The transcriptome will be useful to understand the lipid metabolism pathway and obtain the engineering economic algae species aimed at biodiesel production.

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Sorted by citations

Authors
Yang, F., Xiang, W., Li, T., & Long, L.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
Long, Lijuan
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2018-11-06
Volume
8
Issue
1
Pages
16420-16420
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-018-34650-x
PMC
PMC6219579
OpenAlex
W2899389454
Cited by
109
References
4
Citations by year
2026: 16 · 2025: 20 · 2024: 19 · 2023: 13 · 2022: 16 · 2021: 13 · 2020: 8 · 2019: 4
FWCI
1.44
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
the Strategic Priority Research Program of the Chinese Academy of Sciences
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
phosphorus deficiency, lipid accumulation, Scenedesmus, microalgae, transcriptome, fatty acid synthesis, triglyceride synthesis, starch biosynthesis, carbohydrate metabolism, pyruvate kinase, diacylglycerol acyltransferase, carbon flux, biodiesel, differentially expressed genes
MeSH terms
Phosphorus, Starch, Stress, Physiological, Gene Expression Profiling, Scenedesmus, Lipid Metabolism

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